| OBJECTIVE: In the present study with streptozotocin-induced diabetes in Wistar rats as objects, we have investigated the protective effects of Clau on the behavior profiles and molecular biology aspects.METHODS: The diabetic rats model were produced by injection 48mg/Kg streptozotocin,which were treated by clausenamide. after 3 months, we observed the learning and memory potential by Morris water maze, RT-PCR was used to detect mRNA expressions of HO (HO-1 and HO-2) and COX-2 in hippocampus of diabetic rats with learning and memory deficits.RESULTS:â‘ Compared with control group, the levels of blood sugar andglycosylated hemoglobin in 3-month diabetic rats were increased obviously(P<0.01) , however, the levels of blood sugar and glycosylated hemoglobin ofClau-treated group (50,25mg/kg) were not changed compared with diabetesmodel group (P>0.05) .â‘¡The results of Morris water maze showed that thetime passing through the aim area was prolonged, the frequency passing throughthe aim area was decreased, the time and journey at the first quadrantic werealso decreased (P<0.01) on d4 and d7 compared with normol control, theseprofiles of Clau-treated group (50,25mg/kg)were obviously increased (P<0.01) compared with diabetic group.â‘¢The results of RT-PCR showed that the expressions of HO-1,HO-2 and COX-2 mRNA in hippocampus of diabetic group was higher than that of control group (P<0.01) . The expressions of HO-1,HO-2 and COX-2 mRNA of Clau-treated group (50,25mg/kg) were lower than that of diabetic group (P<0.01) .â‘£The results of immunohistochemistry showed that the expressions of HO-1 and COX-2 in hippocampus of diabetic group were obviously increased, but Clau could inhibit the expressions of HO-1 and COX-2.CONCLUSIONS: Clausenamide can protect learning and memory deficits that follow long-term diabetes. The inhibition of expressions of H0-l,H0-2 and COX-2 in hippocampus of the diabetic rats may account for the protective effects.
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