CD34~+ Cells' Culture And Study Of Their Characters In GFP Transgenic Mice

[Objecvtie]:To approach HSCs bionomics and relation between bFGF,PDGF expression and neoplastic hematologic disorders,detect bFGF,PDGF expression in the GFP transgenic mice marrow mononuclearcells.[Methods]:To culture the bone marrow cells from GFP transgenic mice in vitro.After identified by anti-CD34 monoclonal antibodies,we assembled respectively HSCs in the 1d,5d,7d,10d of culture and detected expression condition of bFGF and PDGF gene by reverse transcription polymerase chain reaction(RT-PCR).[Results]:1.On the 7th day,the rates were CD34+ cells＞76%and to the peak.2.The expression of bFGF and PDGF mRNA were all positive in the 1d,5d,7d,10d of culture by RT-PCR..3.The expression of bFGF and PDGF were the peak at the 7th day,and begun to go dowm at the 10th day in the gray scale measurements.[Conelusions]:1.We could get more CD34~+ cells culturing the suspension cells from GFP transgenic mice bone marrow after abstracting at the 12th hour.2.bFGF and PDGF mRNA were stably expressed in the GFP transgenic mice marrow mononuclearcells and reach at the 7th day,and correlated directly with the CD34~+ cells in the culture.3.bFGF benefitted to HSCs growth during the cultural course;PDGF promoted cells disintegration and generation.