PI3K/Akt Inhibitor Influence On Radiosensitivity Of Adenocarcinoma Of Lung Cancer Cell At Xuanwei, Yunnan Province

PurposeTo investigate the radiosensitive effect of PI3K/Akt's specific inhibitor-Ly294002 on adenocarcinoma of lung cancer cell at xuanwei,yunnan province.Materials and methods1.Clonogenic survival assay was performed to determine survival fraction of XWLC-05 cell at different doses.The parameters D₀,D_q,N,SF₂ and SER from multi-target hit mathematical model were calculated to evaluate radiosensitivity of the cells and were fitted cell survival curve.2.Tetrazolylazo-colorimetry was detected to inhibition rates of XWLC-05 cells as different concentrations of Ly294002 after 24 hours and 48 hours with 4Gy irradiation,Cell radiosensitivity was evaluated.3.Flow cytometry was performed to check the impact on the XWLC-05 cells cycle and apoptosis on different conditions of Ly294002 in order to evaluated the mechanism of Ly294002.Results1.Clonogenic survival assay:The XWLC-05 cells's survival fraction of the control group in different points were higher than experimental group.The control group's values of D₀,D_q, SF₂ were higher than the experimental group.The value of SER is 1.05.The survival fraction between the control group and the experimental group were significantly different(p＜0.05).2.Detection of MTT colorimetric assay:With different concentrations(5,10,20,50μmol/l), inhibition rate of the control group inhibition rate was less than that of the experimental group after 24 hours and 48 hours.By the data t test group,the inhibiting results processed for 24 hours with Ly294002,between the control group and the experimental group were significantly different(p＜0.05).The cells are processed for 24 hours with Ly294002 are of statistical significance(p＜0.05).The inhibition were processed for 24 hours by Ly294002,the control group and the experimental group were of no statistical significance(p＞0.05).3.Detection of the Flow cytometry assay:Having processed for 24 hours with Ly294002, the G₂/M phase cell ratio of the experimental group of no irradiation was greater than the control group,the G₂/M phase cell ratio of the experimental group of irradiation wasless than the control group(p＞0.05).Having processed for 24 hours and 36 hours with Ly294002,the G₂/M phase cell ratio of the experimental group of irradiation was more than the control group(p＜0.05).The result of apoptosis was not obvious after the cells were processed for 12 hours,24 hours and 36 hours with Ly294002,the control group and experimental group apoptosis result there was no significant difference(p＞0.05).Conclusions1.Ly294002 have an radiosensitive effect on XWLC-05 cell.2.After 24 hours,the role of different concentrations Ly294002 inhibited on XWLC-05 cells was in a dose-compliance.With the increasing concentration of Ly294002,inhibition rate increase.Comparing with the control group,the inhibitory rate of the irradiated group was more greatly increasing;The best time processing with Ly294002 may be 24h.3.The G₂/M phase of XWLC-05 cells was blocked after irradiating,the G₂/M phase cells block were increased by Ly294002,The rate of apoptosis was not obvious,It was suggested that the mechanism of Ly294002 on XWLC-05 cell line's radio-sensitizing effect may be blocking cells during G₂/M phase.