Heat Shock Transcription Factor1 Gene Silenced With RNAi To Enhance Radiosensitivity Of Cervical Carcinoma Cell

To suppress HSF1 gene expression using RNA interference technique to explore the changes of the radiation sensitivity of cervical carcinoma cells.Methods: Construct pSilencerHSF1siRNA plasmid expression vector, plasmid expression vector which could express siRNA was transfected into cervical carcinoma cells by lipofectamine. HSF1mRNA expression was detected by Real time polymerase chain reaction. Flow cytometry was employed to evaluate HSF1protein expression. In addiction the radiction sensitivity of cervical carcinoma cells was assessed by clone forming assay.Results: After evaluation and sequencing, the plasmid expression vector was set up successfully. pSilencerHSF1siRNA plasmid expression vector can suppress HSF1 gene expression specifically, and inhibition rate of HSF1 expression was 80%-90%. The result of flow cytometry showed that, HSF1 protein in Hela cells could be knocked down specifically by HSF1-pSilencer2.1-U6 vector. The condition of HSF1 gene silence, the survival rate of cells which was in RNAi was much lower than control group by cloning.