Preparation And Physic-chemical Properties Of Silk Fibroin/Hyaluronic Acid Blend Material

The blend film and porous material of silk fibroin(SF)/ hyaluronic acid (HA) were prepared by blending regenerated Bombyx mori silk fibroin solution and hyaluronic acid solution. This paper takes a research on the relationship between preparation conditions and physic-chemical properties, trying to provide a new material in biomedical field.The SF/HA blend films were prepared by casting SF/HA mixture solution on a polyethylene plate and drying with hot air. The condensed structure of blend films was characterized by XRD, FT-IR. The water solubility, broken tensile strength, broken elongation and Young's modulus of blend films were tested and analyzed. The L929 fibroblastic cells were also cultrued on the blend films. Results indicated that there were mainly random coil in the uncross-linked blend films, HA interfered SF to form crystal structure, whereas 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) stimulated SF to form Silk I. EDC had a remarkably cross-linking effect on the SF/HA blend films and the water solubility of the blend films decreased obviously. Compared with the uncross-linked SF/HA blend films, broken elongation of cross-linked blend films increased remarkably while broken strength and Young's modulus decreased, the flexibleness of the films enhanced obviously. The change was especially remarkable when the proportion of HA smaller. SF/HA blend films can support the growth of L929 fibroblastic cells.SF/HA blend porous materials were prepared by freeze-drying. The condensed structure of blend porous materials was characterized. The physic-chemical properties such as water solubility and pore structure were tested and analyzed. The results showed that condensed structure of uncross-linked SF/HA blend porous materials were mainly amorphous domains. EDC also had a remarkable cross-linking effect on the SF/HA blend porous materials. The pore diameter decreased with the increasing of proportion of HA and the falling of freezing temperature. The porosity and water-uptake increased with the rising of freezing temperature and increasing of the proportion of HA. By adjusting the technical condition, the SF/HA blend porous materials can be prepared with the average pore diameter from 55.22μm to 383.82μm, the porosity from 74.1% to 96%.