Effect Of Autologous Mesenchymal Stem Cells Mobilization On Inflammation And Remodeling Of Ischemia Cardiomyopathy

OBJECTIVES: To study the the inflammation and remodeling after mobilization autologous bone marrow mesenchymal stem cells at different time points in myocardial infarction models, and to explore the mechanism initially.METHODS: 20 healthy Taihu Meishan swines were divided into four groups randomly to build the model of myocardial infarction. Group A: control group. Group B: transplanted MSCs through coronary artery 3 hours after myocardial infarction. Group C: treated with G-CSF after myocardial infarction through intravenous injection. Group D: injecteed G-CSF for 5 days 1 week after MI. The mesenchymal stem cells were pre-cultured. IL-1β,IL-10,MMM-9 and Ang-II numerus of blood serum were observed in different periods. The change of left ventricle pressure was detected with Powerlab and Millar catheter. We also observed the change of each index of cardiac function with MRI. Specimen was got at the end of experimental time, then access to the data of myocardial infarction.RESULTS: Compared with other groups, the index of group C improved significantly including LVDP, + dp/dtmax and - dp/dtmax and EDWT, SV,aslo, there was significant difference as to the area of myocardial infarction at the end of experimental time. Serum levels of IL-1β,MMP-and Ang-II in group C dropped significantly compared with other groups. At the same time, IL-10 in C group elevateed significantly.CONCLUSIONS: The therapeutic effect of group C was more obvious than other groups lied on the regulation between Anti-inflammatory cytokines and Pro-inflammatory cytokines in early time and inhibited the progress of ventricular remodeling in the early and late time. In addition, group C could reduce the area of myocardial infarction and increase cardiac output as well as induce left ventricular thickening after MI.