| Objective: The dynamic expression of angiopoietin1(Ang1)and its receptor Tie2 were investigated inβ-ray radiation injury of skin wounds, for in-depth study ofβ-ray skin injury wound healing mechanism difficult to provide a theoretical basis.Methods: Fifty-four male SD rats were divided into 3 groups.The model of acute radiation injury of skin wound in rats was replicated by 45 Gyβ-ray to the skin radiation (R,n = 24); The copper column with 30 mm diameter will be placed in boiling water for 15 minutes after removal in the absence of outside pressure placed under the skin of the hip eight seconds, to establishment the model for deep second-degree burns(B,n=24);and 6 normal rats were served as normal control group(N,n=6).From different periods skin wounds,the expression of Ang1 and Tie2 protein and Ang1 mRNA /Tie2 mRNA were respectively semi-quantitative checked by means of immunohistochemical tcchnique SP and reverse transcription PCR( RT-PCR).Results:①Lower expression of Ang1 and Tie2 was identified in the rat skin in normal control group.②The Ang1 value of the absorbance in the radiation group was 0.130±0.012,0.170±0.013,0.192±0.072,0.213±0.061,0.225±0.0122;the burn group value of Ang1 was 0.190±0.182,0.332±0.014,0.273±0.014,0.225±0.013,0.175±0.052. There was statistical significance between the two groups(P<0.05).③The Tie2 value of the R group was 0.112±0.011,0.183±0.012,0.171±0.061,0.203±0.014,0.261±0.054 ;and the B group was 0.170±0.062,0.310±0.013,0.284±0.014,0.213±0.012,0.152±0.031, There was also statistical significance between the two groups(P<0.05).④The results of RT-PCR for Ang1 mRNA /Tie2 mRNA were in line with the results of the immunohistochemistry. Conclusion: The continuing low expression of Ang1 and Tie2 may be partially involved in the mechanism of retarded healing of acuteβ-ray radiation injury in skin wounds.
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