The Expression Of Glucose Regulated Protein 78 And Its Significance In Resistance To Cisplatin In SPCA-1 Cell Line

Objective: In this study we are the first to investigate the expression of GRP78 under the induction of A23187 and its significance in the resistance to anti-tumor drugs cisplatin in a human lung cancer SPCA-1 cell line.Methods: RT-PCR and Western Blotting were used in analyzing the expression of GRP78 at mRNA and protein levels in SPCA-1 cell line induced by A23187 at different concentration (0,1,2,4,6μM ). Cell survival to cisplatin was determined by MTTResults: The expression of GRP78 at both mRNA and protein levels were increased obviously in SPCA-1 cell line induced by A23187, and A23187's treatment caused a dose-dependent increase of GRP78 to a great degree. The results of MTT shows that there is significant difference between the group with 1μM and control group, However, for groups of A23187 at concentration of 2,4,6μM,the cell survival decrease ,there was a dose-dependent in cell survival.Conclutions: The expression of GRP78 at both mRNA and protein levels were increased obviously in SPCA-1 cell line induced by A23187,which associated with the survival of the cell.We conclude that there is correlation between the expression of GRP78 and resistance to cisplatin.