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Staphylococcus Aureus Resistant In Milk And Toxic Properties Of Enterotoxigenic Research

Posted on:2009-12-11Degree:MasterType:Thesis
Country:ChinaCandidate:D HuangFull Text:PDF
GTID:2144360245965918Subject:Food Science
Abstract/Summary:PDF Full Text Request
Received a total of 75 samples from Huhehaote five sampling ranch during 2007.From the 75 samples that 25 Staphylococcus aureus were separated. At the same time, relatively resistant. Staphylococcus aureus enterotoxins for the extraction,, determination and sub-type identification.The results show that: From 75 separate samples to identify 25 of Staphylococcus aureus screening of the paper are 10 relatively resistant to erythromycin, streptomycin, penicillin, ampicillin strains, And that 10 of the 10 kinds of antibiotics (Amikacin,Ciprofloxacin,Penicillin,Erythromycin,Cephazolin,Ofloxacin,Streptomycin,Ampicillin,CefotaximeSodiium,Sulfame) to the sensitivity of tests, That strain SA18, SA20, SA25 of ofloxacin moderately sensitive; Strain SA5, SA18 moderately sensitive to ciprofloxacin, strain SA2 of Cephazolin of sensitive forest, Strain SA5 of cefotaxime resistance, The remaining strains are sensitive to the cephalosporin class of antibiotics.On the other selected antibiotics show some patience。At the same time, 10 Staphylococcus aureus enterotoxins to extract, Determination and classification study, By calculating that the final 10 extracts of Staphylococcus aureus enterotoxins contents were . Isolation and identification of 10 Staphylococcus aureus in, Only one had produced a toxin, accounting for 10%(1/10), Intestinal toxins produced two kinds of strains have seven, accounting for 70%(7/10), Produce three kinds of intestinal toxins are two strains, accounting for 20%(1/5), Which produce the toxin type A strains have had four, accounting for 40%(2/5), Which produce the toxin type B strains have had three, accounting for 30%(3/10), Which produce the toxin typeD strains have had four , accounting for 40%(2/5), Which produce the toxin type E strains have had three , accounting for 30%(3/10). Finally, the intestinal toxins in the highest growth curve SA23 strain to the determination of the 18 h at the growing number.
Keywords/Search Tags:Staphylococcus aureus, Antibiotics, Resistance, enterotoxin
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