| Objective:In the current study, we investigated the effect of pravastatin and atorvastatin by examing the changes of matrix metalloproteinase-2 (MMP-2) , interleukin-6 (IL-6), ventricular remodeling and heart falure after MI. To clarify the new knowledge of ventricular remodeling and provide a novel method for its therapeutics.Method: we established the left ventricular infarct in rats by ligation of the left anterior descending coronary artery. Healthy male Wistar rats (12-15 weeks and 200-250g) in this study were divided into MI group(n=15), MI+Statin group (n=30) and Sham group(n=8). MI+Statin group were divided into pravastatin group(n=15) and atorvastatin group(n=15). The MI models were established by ligating the anterior descending coronary artery; sham group was given the same procedure but without ligation. After 8 weeks, 6 randomly selected rats in each group received ultrasound cardiography and hemodynamic parameters, then killed to examine body weight (BW), left and right ventricular weight(LVW,RVW). Collagen volume fraction(CVF) was measured using Van Gieson(VG) in non-infarct region. The expression level of MMP-2 and IL-6 were detected by ELISA method.Results:1.Those dead, infracted area less than 30% or more than 55% and not having integrated datum AMI rats were excluded, and finally there were 8 rats in MI group, 10 in pravastatin group, 9 in atorvastatin group and 8 in Sham-operated group. There was no difference in infarcted area among the four groups (40.02%-44.70%).2.MMP-2 levels in blood were higher in the MI and MI+Statin groups than Sham group (P<0.05), but MI+Statin group is lower than MI group (P<0.05).3.Serum IL-6 levels in MI and MI+Statin groups were higher than Sham group (P<0.05), but MI+Statin group is lower than MI group (P<0.05).4.RVW/BW,LVW/BW,LVEDP measurements were higher in the MI and MI+Statin groups than Sham group (P<0.05), but MI+Statin group is lower than MI group (P<0.05).5.Compared with MI group, CVF number in MI+Statin group was lower(P<0.05).6.Compared with Sham group, left ventricular end-diastolic diameter(LVEDD) increased, but fractional shortening(FS) and left ventricular ejection fraction(LVEF) decreased in MI and MI+Statin groups(all P<0.01). the results were to the opposite when MI+Statin compared with MI group.7.In all measurements examininged above, there was no difference between pravastatin and atorvastatin groups (p>0.05).Conclution:Our research demonstrate that statins have the effect of decreasing MMP-2, IL-6 and repressing destruction of the collagen network and deposition of redundant reactive collagen, thus reverse left ventricular remodeling and improve cardiac function. There was no significant difference between pravastatin and atorvastatin groups.1.Manifestations of late ventricular remodeling after MI includes LV dilatation, myocardium thickening and spherical shape change,interstitial fibrosis, increased protein expressions of MMP-2 and IL-6 , ventricular function deterioration.2.Statins can effectively decrease collagen deposition and expression levels of MMP-2,IL-6 inhibit dilatation, lessen protein reactive thickening and improve cardiac function.3.There was no significant difference in the effect between pravastatin and atorvastatin.
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