| Objective: To detect the expression of anti- M2-muscarinic receptor autoantibody in serum of asthma model rats. To clarify the correlation of anti-M2-muscarinic receptor autoantibody to the development of asthma. Through injecting cyclosporin A(CsA) in abdominal cavity, to confirm effective therapy contribution of Cyclosporin A about asthma.Methords: Thirty-nine male healthy wistar mice were divided randomly into 3 groups: group A as control, group B allergized with ovalbumin (OVA), group C treated with Cyclosporin A. serum anti- M2-muscarinic receptor autoantibody was detected with SA-ELLISA immunoassay, leukocytes amounts,eosinophil(EOS) amounts,eosinophil percentage in BALF were counted under microscope,and airway pressure time index(APTI) were measured.Result: The positive ratio of anti- M2-muscarinic receptor autoantibody(83.33%) and antibody titer(1:120) in serum was more in asthma model rats than control group(8.33% ; 1:20) and CsA group(25.00% ; 1:35). leukocytes amounts(35.34±0.77×104/ml),EOS amounts(8.29±0.44×104/ml),eosinophil percentage(23.45%±0.77) of BALF in asthma model group were more than in control group (8.44±0.39×104/ml ; 0.06±0.01×104/ml; 0.70%±0.06) and CsA group(24.43±0.67×104/ml ; 2.80±0.15×104/ml ; 11.44%±0.31). When the concentration of mACh is 0.5mg/kg , 1.0mg/kg , 1.5mg/kg , APTI in asthma group (0.504±0.143mmHg/s , 0.617±0.108 mmHg/s , 0.794±0.073 mmHg/s )was significantly higher compared with control group(0.277±0.161 mmHg/s , 0.434±0.128 mmHg/s , 0.463±0.120 mmHg/s) and CsA group(0.416±0.150 mmHg/s , 0.541±0.117 mmHg/s , 0.676±0.092 mmHg/s).Conclusion: The positive ratio of anti-M2-muscarinic receptor autoantibody and antibody titer were much higher in asthma model rats than that from the control group and decreased after injecting cyclosporin A. Anti-M2-muscarinic receptor autoantibody may play a important role in the pathogenesis of asthma. CsA can reduce airway hyperresponsiveness and airway inflammation in asthma model rats.
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