| Objective To observe the effect of remifentanil preconditioning and ischemic preconditioning on cell apoptosis,Bcl-2 and Bax protein induced by ischemia-reperfusion injury in rat liver tissue and the diffence of two methods.Methods Seventy-two healthy male SD rats weighing 220-250g were randomly divided into 4 groups(n=18 each):group S were the group of sham operation,group IR were infused with normal saline before ischemia,group IPC received 3 episodes of a 5 min ischemia followed by a 5 min reperfusion before ischemia.Group RPC were infused with remifentanil 2μg·kg-1·min-1 30 min before ischemia.The model of partial liver ischemia/reperfusion was used.The reperfusion was performed after 30 min ischemia in the lives.The animals were killed at 1,3,5h reperfusion and left liver tissues was sampled.Light microscope and Electron microscopy was performed to verify the morphologic changes of apoptosis.Apoptosis index were analyzed by TUNEL..The expression of Bcl-2 and Bax protein were observed by immunohistochemical technique.Results There were no significant apoptosis changes in Sham group.Apoptosis index and Bax protein in group IR increased significantly as compared with that in group S(P＜0.05).Bcl-2 protein in group IR prominent decreased compared with Sham group(P＜0.05).In contrast with IR group,Apoptosis index in IRC and RPC groups are lower(P＜0.05);Bcl-2 protein in IRC and RPC groups are higher(P＜0.05),and there is no difference in IRC and RPC groups(P＞0.05);Bax protein in IPC group are a litter lower(P＞0.05);Bax protein in RPC group are lower(P＜0.05).Light microscope and electron microscope showed that apoptosis of liver tissures was attenuated by remifentanil preconditioning and ischemic preconditioning.Conclusin Ischemic and remifentanil preconditioning can reduce cell apoptosis in early stage of reperfusion of rat liver.RPC can inhibit apoptosis by decreasing Bax and increasing Bcl-2 expressing and IPC by increasing Bcl-2 expressing.
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