The Study On The Correlation Between MSX1 Gene Exon2 And 3'non-coding Region Mutation And NSCL/P

Objects:1,Study the change of the expression of MSX1 gene in the NSCL/P and in the normal.2, Research relevance of MSX1 gene mutation and NSCL/P pathogenesis in Shan-xi China.Methods:Collecting the samples.Collecting the oral mucosa from the fissures of 30 children with NSCL/P and 30 normal control infants,then the mucosa was rapidly frozen in liquid nitrogen, the RNA was extracted with the RNA Extraction Kit from the company TAKARA.The expression of MSX1 gene.According to the NCBI database,picked out the MSX1 gene sequences(serial No:NM₀02448) and designed the primers.Using PCR-PCR technology and the RNA extracted from the mucosa as a template,according to the Central Dogma supplementary theory,amplify products of MSX1.Then the expression of MSX1 gene between the NSCL/P and the noemal person was compared.Picking up the variants.Compared the results of each sequence with the MSX1 normal sequence provided by the Genbank and found the variants.A case-control study of the variability of each gene frequencies were tested.Results:The expression of MSX1 gene.Quantification of the expression levels,a statistical analysis showed that the result in patient group and control group were respectively(29.73±19.729)and (41.97±26.355),the patient group was lower than the control group(F=-2.783,P=0.007＜0.05).Picking up the variants.MSX1 gene exon 2 and 3 'un-translated region was amplified to compare the sequencing,the result revealed that there were five significant variants,including the X₁₇(TT/TC886) P=0.000＜0.05,X₂₅(AA/AT904) P＜0.05,X₂₆(GG/GA912) P= 0.002＜0.05,X₂₉(GG/GA1389) P=0.011＜0.05,X₃₀(GG/GAA1389) P=0.000＜0.05.Using the Logistic regression analysis,regression coefficient of 2 variants in 5 was -3.738, -22.181 respectively.Conclusion:There was correlation between the MSX1 mutation and the pathogenesis of NSCL/P.