Effects Of Shenqi Injection On Ventricular Remodeling In Rats With Congestive Heart Failure

ObjectiveTo investigate the effects of Shenqi injection on ventricular remodeling and cardiac function of congestive heart failure rats induced by Adriamycin, which to provide evidence for clinic.Methods:Utilizing cardiac toxicity of Adriamycin to make the model of congestive heart failure rats. Sixty male SD rats were divided randomly into normal group, adriamycin model group, captopril group, Shenqi injection(short by Shenqi following) low dose group, Shenqi middle dose group, Shenqi large dose group. The spirits, activities, eatings were observed during the lab proc. Eight weeks later, we determined rat left ventricle heart indexs, pathological changes of cardiac muscle by HE dyeing, expressions of collagen proteinâ… ,â…¢in cardiac muscle by immunity class, semiquantitative analysis of MMP-3, TIMP-1mRNA in cardiac muscle by RT-PCR methods.Results:1. Cardiac function determination: the cardiac function of model group were lower than the normal group (P<0.01). Compared with model group, each drug treatment group could improve cardiac function in different degree. Among these groups, the curative effect of Shenqi middle dose group was significant(P<0.01), the curative effect of Shenqi large dose group was unknown significance(P>0.05).2. Myocardial hypertrophy index: the index and heart form of model group were highed than the normal group(P<0.01). Compared with model group, each drug treatment group could improve myocardial hypertrophy in different degree. Among these groups, the curative effect of Shenqi middle dose group was significant(P<0.01), the curative effect of Shenqi large dose group was unknown significance(P>0.05).3. The expression of collagen proteinâ… ,â…¢: the results of immunity class dyeing showed that the expression of collagen proteinâ… ,â…¢in group model group were all obviously higher than normal group(P<0.01);compared with model group, each drug treatment group could decrease the expression of collagen protein. Among these, the expression of Shenqi middle dosage group were significantly decreased(P<0.01), the expression of Shenqi large dosage group were unknown significant difference(P>0.05).4. The results of RT-PCR:compared with normal group, MMP-3mRNA of model group were significant increased(P<0.01). TIMP-1mRNA of above group were significant decreased(P<0.01).The expression of MMP-3mRNA in small and middle dosage group and captopril group were significant lowed than the model group(P<0.01, P<0.05).The expression of TIMP-1mRNA in above group were significant highed than the model group(P<0.01, P<0.05). The expression of MMP-3mRNA,TIMP-1mRNA in large dosage showd no significant difference(P>0.05).Conclusion:1. Shenqi injection can improve the cardiac function.2. Shenqi injection can lessen the pachynsis of left ventricle in congestive heart failure rats.3. Shenqi injection Can improve the expression of collagen proteinâ… ,â…¢.4. Shenqi injection can decreased the expression of MMP-3 or inhibit its activity, and increased the expression of TIMP-1.