Experimental Research On Effects Of Anti-liver Fibrosis Of Octreotide Targeting At Hepatic Stellate Cells

Objective: To investigate the main mechanisms of anti-fibrotic action of octreotide. Methods: A rat hepatic stellate cell line, rHSC-99, was treated with octreotide. Cell proliferation was assessed by MTT colorimetric assay;The content of TGF-β1,type I,III collagen was examined by ELISA;RT-PCR was used to detect mRNA of MMP-2,TIMP-1,collagen type I and collagen type III.Results: 1. Octreotide could significantly inhibit the in vitro rHSC-99 proliferation (P <0.05),this inhibition in a certain range in a dose-dependent.2.Octreotide presence in the premise,the concentration of TGF-β1,type I,III collagen compared with the control group decreased significantly(P<0.05), and compared with the control group, type I,III collagen mRNA level decreased significantly (P<0.05). 3. Compared with the control group, octreotide intervention group performance for the MMP-2 mRNA expression (P<0.05) and TIMP-1 lower expression (P<0.05).Conclusions: Octreotide could inhibit the proliferation of HSC, could lower type I and type III collagen mRNA and protein expression,and could inhibit the fibrosis factor of TGF-β1 protein secretion,by inhibiting the expression of MMP-2 and TIMP-1 level, improve the imbalance of the synthesis and degradation of ECM,suggesting that octreotide might play a role in the anti-liver fibrosis and to reverse the role of hepatic fibrosis targeting at hepatic stellate cells.