Studies On Pharmacognosy And Fingerprint Of Celastrus Orbiculatus Thunb

Celastrus orbiculatus Thunb. is one of folk medicine and distributes widely in China. It was used for activating blood, detoxification and detumescence.The extracts of Celastrus orbiculatus Thunb. have various activities, inculding anti-inflammatory, anti-tumorand, immunosuppressive, etc. Its preparation was used for the treatment of rheumatoid arthritis, and other autoimmune diseases in clinical.Celastrus orbiculatus Thunb. was reviewed in its distribution, application, chemical components, pharmacological function, clinical applications, etc. And also, its pharmacognostic properties, main contents and quality control were studied.In this thesis, both dulcitol and pristimerin, the primary contents in Celastrus orbiculatus Thunb., have been studied by Thin Layer Chromatography (TLC). The method may help to establish the quality standards for Celastrus orbiculatus Thunb. and its preparation.The method of content determination was established by chromatographic experiments. The results showed the method was easy to operate and accurate. Content of dulcitol from Celastrus orbiculatus Thunb. was determined by HPLC method. The results showed that the contents of dulcitol from Celastrus orbiculatus Thunb. in different producing area revealed a sharp difference, and this result indicated the quality control of Celastrus orbiculatus Thunb. should be improved.The Chromatographic Fingerprint of its bioactive component was established by HPLC method. The methods were stable, reliable and specific for analysis bioactive component from Celastrus orbiculatus Thunb. The method can be applied to the quality control of Celastrus orbiculatus Thunb. In addition, as a further research, the relationship between the bioactive components group and their effects, was necessary and important.Moreover,The FTIR spectra of Tripterygium hypoglaucum (level) Hutch (THH) and Celastrus orbiculatus Thunb are studied. The result show that the two herbs have different ratio of main substance calcium oxalate and dulcitol, so their absorption frequency and form are different as well. There is an obvious different between Celastrus orbiculatus stem and root,both the peak form of calcium oxalate and dulcitol in the FTIR spectra of roots are sharper and stronger than stems. Therefore FTIR can be a fast and simple method to indentify Celastrus orbiculatus.