Studies On Association Of GM130 Gene Exons And Interleukin-6 Gene Polymorphisms With Gastric Cancer Risk In Northwest Chinese Population

Background: Gastric cancer(GC) is one of the most common cancers and the second leading cause of cancer death in the world. The underlying pathogenic mechanisms have not been fully understood, although the interactions between environmental, bacterial and multiple genetic components are likely to be involved. The present study was undertaken to investigate association of GM130 gene exons and IL-6 -174 base genetic polymorphisms with gastric cancer in northwest Chinese population, to provide evidence for the early diagnosis, therapy and prevention of GC.Methods: 142 patients with Gastric cancer(GC) and 200 healthy controls from Northwest China have been genotyped for GM130 exons 1-5 by using polymerase chain reaction-denaturing high performance liquid chromatography (PCR-DHPLC) and IL-6 -174 base by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The analysis of their genotypes and allele distributions is performed.Results: No polymorphism in exons 1,2,3,4 and 5 of GM130 was found in Chinese population from north-western China. The G/G genotype of IL-6 -174 base is 100% in gastric cancer group and 99% (198/200) in control group. There are no G/C and C/C genotype in gastric cancer group. The G/C genotype is 1% (2/200) in control group. The genotype and allele distributions of IL-6 -174 polymorphism was not significantly differences in GC patients compared with the controls(P>0.05). Conclusions: No significant differences were found in GM130 gene exons 1-5 and IL-6 -174 base between GC and control groups. The mutations maybe are not the nosogenesis of GC.