â… .Solution Structure Of Bip By NMR Study And The Effect Of Bip For Guinea Pig Ileum Contraction Induced By Bradykinin â…¡.Synthesis Of Mono-O-(3-cholesteryloxycarbonyl) Ropionyl Polyethylene Glycol And Mono-O-(3-cholesteryloxycarbonyl) Propionyl Poloxam

Bradykinin not only participates in a large number of physiological and pathological processes, such as vasodilatation, increasing vascular permeability, control of blood pressure and production of pain, but also participates in several inflammatory processes. Apart from these effects, bradykinin has shown to induce potent contractile or relaxant effects in several vascular and nonvascular smooth muscles. Some peptides, called ACEI/BPPs, seprated from venoms have many specific activites. ACEI/BPPs are comprised of 5-14 amino acid residues, usually with a pyroglutamic acid residue at N-terminus and rich prolines. They have the ability to inhibite angiotensin - convertion enzyme and potentiate the activity of bradykinin.Two dimensional nuclear magnetic resonance (2D NMR) is one of the main methods for studying the structure of peptide and protein. It can gain many spatial information of peptide via chemical shift of amino acid residues protons and coupling action among protons in COSY, TOCsY and NOESY. Then one can do structure calculation of physiological state with computer by some sofewares.The bradykinin inhibiting peptide (BIP) was synthesized by Fmoc solid phase peptide synthesis, then was purified by high performance liquid chromatography and characterizated by mass spectroy. Through DQF-COSY, TOCSY and ROESY, the amino acid spin system and the sequence recognization were integrated. The analogic spatial structure of BIP was obtained by CNS.By the isolated guinea pig ileum contraction test, we knew that BIP can inhibit the guinea pig ileum contraction effect induced by bradykinin. Additionally, we knew that BIP could induce contraction of guinea pig ileum and the contraction was noted with increasing concentrations.Cholesterol is an important constituent in life and its derivatives have a wide application. Polyethylene glycol and poloxamers are amphiphilic polyether with high biocompatibility. We described a route for the conjugation of cholesterol to polyethylene glycol and poloxamers with succinate, which improved the hydrophilicity and resulted in amphiphilic. The chemical structures were analysized with FT-IR and 400MHz ~1H NMR.