Experiment Of SASP On Renal Interstital Change In Rat After Unilateral Ureteral Obstruction

Backgrounds:Renal interstitial fibrosis is not only the common pathway of all kinds of chronic renal diseases but also the main Pathological change resulting in end stage renal diseases.Various Primary or secondary renal diseases can develop renal interstitial fibrosis,and finally lead to renal function failure.It is very complicated for the mechanisms of renal interstitial fibrosis,which may be involved with a lot of causes such as related cells,cytokines,extracellular matrix and so on.NF-κB Plays an important role in fibro genesis of different tissues or organs including kidney. Sulfasalazine(SASP),as a specific inhibitor of nuclear factor kappaB.The mechan ism of sulfasalazine treating renal interstitial fibrosis is unknow.For this reason,we try to investigate the mechanism of Sulfasalazine treating renal interstitial fibrosis in this experiment basing on our previous study.Objective:To observe the expression of NF-κBp65 and TGF-β1 in the obstruc -tive renal tissue of UUO rats intervening by sulfasalazine,and explore it'smechanism in preventing the Progress of renal interstitial.Method:Forty-two Sprague-Dawley rats are randomly divided Into three groups:sham-operated rats group(SOR,n=14);unilateral ureteral obstructive model group(UUO,n=14),SASP-treated group(SASP,n=14).Group UUO and group S ASP are all under the operation of unilateral obstruction.The ureter is dissociated but not ligated in the group SOR.Group SASP is treated with SASP(100mg.kg^-1.d^-1)after operation.The left two group are treated with distilled water of equal volume.Seven rats of each group are sacrificed at the 7th,14th day,after operation.The obstructive kidneys are taken out.The tubulointerstitial damage index are observed by HE, Masson stain.The expression of NF-κB p65 and TGF-β1 in renal of mesenchyme are detected by imunohistochestry.Results:1.The tubulointerstitial damage index of HE and Masson stain is increased with time dependence in group UUO,which is obviously higher than group SOR at each time Point(P＜0.01).It can be decreased after treating by SASP at each time Point(at the 7th,14th day,P＜0.01).2.The expression of NF-κBp65 and TGF-β1 are increased with time dependence in group UUO,which is obviously higher than group SOR at each time Point(P＜0.01),The expression of NF-κBp65 and TGF-β1 are little at each time Point in group SOR.They can be decreased after treating by SASP at each time Point(P＜0.01).Conclusions:1)In comparison with sham- operated group tubular lesion, interstitial fibrosis in UUO and treatment groups markedly increased.The kidney tubular pathological changes were aggravated along with the obstruction time.2) Treated with SASP may be reduce or prevent development of early renal interstitial fibrosis by inhibit the expression ofNF-κB p65 and TGF-β1.