| ObjectiveLeukocyte rheology is probably playing an important role in the physiology mechanism of leukocytal function, so my work focused on the effects of rheological properties of leukocytes in rats in terms of cellular Rheology. To interpret the deformability, chemotropism and adhesiveness of Leukocyte, It can help us not only know leukocytal function further, but also can provide a new theory for early diagnosis and clinical treatment of intracerebral hemorrhage.Methods:1 To make an intracerebral internal capsular hemorrhage animal model: 54 male Wistar rats were used in this experiment. They were positioned in a stereo tactic frame and a cranial burr hole (1 mm) was drilled on the right AF0.1-0.2 mm. 100μl autologous blood was sampled and then infused stereotacticlly into the right internal capsular. About sham control rats, they had only a needle insertion. After the infusion, the needle was removed, the burr hole was sealed with bone wax, the wound was sutured, and the animal was placed in a warm box with free access to food and water. Berderson test was done by a single observer without knowledge of neurological condition. Motor behavior was evaluated using testing each rat at 3h, 6h, 12h and 24h after operation.2 Morphological observation of brain : After finishing the Preparation of acute hemorrhage animal model, take the brain tissue and fix it with 10% formaldehyde solution. Through HE strain, observe their dynamic pathological changes under the light microscope.3 Observation of Microcirculation: By use of Microcirculation microscope and computer-image analyses system , pick out the capillary of no branch ,100μm length and 10～30μm diameter, to observe the leukocyte Rheology.4 Statistical analyses: All the data were analyzed by SNK test with SPSS 15.0 statistical software.Results1 The results showed that, the neurological deficit scores were the highest at 12 h and 24h after operation in the Berderson testing method. At all time points, the scores of the Sham-operated rats were lower than that of the ICH rats and significant differences existed between the two groups.2 The lesion of experiment group was severe than that of the sham group which is irregular or ellipsoidal blood clot, different size, rarefaction, edema on the tissue of perihematoma, A few of neurocyte and gliocyte are cellular swelling. Inequality size of vacuole in the cell spaces, and many of Leukocytes infiltrate. These pathological changes are more severity along with the longer time of haematoma formative.3 The Rheological properties of leukocytes in the microcirculation of mesentery in rats were changed obviously in intracerebral hemorrhage group than those of the control group. from 3h to 24h group, the rolling velocity slowed, the number of rolling leucocytes and adherence on endothelium increased, and TLECT obviously extended.Conclusion1 We can copy the model of intracerebral internal capsular hemorrhage by use of stereo tactic method successfully.2 Inflammatory infiltration, neuron necrosis, glial hyperplasia and brain edema in haematoma surrounding of experimental ICH rats may be one of the causes of brain edema and neurobehavioral changes.3 The rheological properties of leukocytes have been changed by intracerebral hemorrhaged stress respond. Microcirculation disturbance is one important pathomechanism of stress ulcer and other disease. The Rheology properties of Leukocytes can impact on microcirculation directly, and the Microcirculation disturbance can impact on leukocytal circulation. The interaction is more and more obviously along with bleeding time. So, study the changes of rheological properties of leukocytes is useful to prevent and treat the complication of brain hemorrhage beforehand.
|
PDF Full Text Request