Treatment And Establishment Of Animal Model Of Abdominal Compartment Syndrome

Objective: To investigate the changes of PCO2, PO2, Cr, BUN and survival rates on the basis of establishment of animal model with abdominal compartment syndrome(ACS). At the same time, we investigate methods of abdominal decompression in ACS include: laparotomize and drainage of abdominal.Methods:New Zealand rabbits, weighting 2.5-3.0kg, were used from Hebei medical university. First, make a simple determinator:40cm length about tubing which was cut from disposable apparatus infusionis connected with the three links and sphygmomanometer. Second, make ACS animal model. Animals of study group were fast. Then they were fixed after intravenous anesthesia. And they were punctured and filled nitrogen in the abdomen. We determined the pressure and maintained the IAP of 30 mmHg 2 hours. Then ACS group was randomly assigned to two groups: laparotomize decompression group and abdominal drainage decompression group. Samples were collected in two hours after abdominal compression and decompression treatment after two hours. The levels of PO2, PCO2, BUN and Cr were measured. Compared the levels of PO2, CO2, BUN and Cr among those study groups. And we compared survival rates between different treatment groups. Results: 1 The design method of animal model is simple, low-cost, and feasible. And it overcomes shortcomings of previous experimental animal model. 2 Comparing with the normal control group(38.63±2.24)mmHg,(104.70±9.86)μmol/L,(6.63±0.61)mmol/L, the levels of PCO2, Cr, BUN significantly increased in ACS group(46.0±2.85)mmHg,(208.19±9.61)μmol/L,(12.29±0.66)mmol/L(P<0.01). Comparing with the normal control group(97.63±1.04)mmHg, the level of PO2 significantly decreased in ACS group( 76.18±3.62 ) mmHg ( P<0.01). 3 Comparing with the laparotomize decompression group ( 41.7±2.52 ) mmHg,(145.3±6.59)μmol/L(,8.30±0.46)mmol/L, the levels of PCO2, Cr, BUN significantly decreased in abdominal drainage decompression group(36.68±2.92)mmHg,(125.36±5.54)μmol/L,(7.08±0.39)mmol/L(P<0.05～P<0.01) . Comparing with the laparotomize decompression group(88.53±3.53)mmHg, the level of PO2 significantly increased in abdominal drainage decompression group(94.12±3.0)mmHg(,P<0.01). Comparing with before treatment, two groups were significantly inproved(P<0.05～P<0.01). 4 The survival rate of abdominal drainage decompression group (93.33%) is higher than the laparotomize decompression group (60.0%), (X2=4.658,P=0.031).Conclusions: 1 The design method of animal model is simple and feasible. It demonstrated that the levels of PCO2, Cr, BUN significantly increased and the level of PO2 significantly decreased in ACS group which compared with the normal control group. 2 The abdominal decompression in the early of ACS can improved acute respiratory dysfunction and renal failure. It demonstrated that the levels of PCO2, Cr, BUN significantly decreased and the level of PO2 significantly increased in ACS group which compared with the normal control group. So it is important that the treatment of abdominal decompression should be early in ACS. Comparison of the two abdominal decompression methods, abdominal drainage decompression is better than laparotomized decompression. It demonstrated that the levels of PCO2, Cr, BUN are lower and the level of PO2 higher in abdominal drainage decompression than those of laparotomize decompression. And it reduced serious pathophysiological damages brought about by the large abdominal operation.