Research Of The Sex Gland Axle Chondriosome Function In Model Rats Of Asthenia Cold Syndrome And The Levels Of Gonadalhormone

Objective: Asthenia cold syndrome have closed correlation with energy metabolish. This experiment means approaching the changes if together with damage of genitor-function of Asthenia cold syndrome , through research the changes of hypothalamus, master gland, ovarian chondriosome function (content of SDH and ATPase) in Model Rats of Asthenia cold syndrome, and its relationship with gonadalhormone. Jinkiu Decoction for warming meridian if effect by adjusting store and dilapsus exploitation two link of sex gland axle energy, to reveal essence of department of gynecology Asthenia cold syndrome, provided theory and experiment evidence to investigation of syndrome of traditional Chinese medicine.Method: Adoption 50 depuratory SD female rats, which first class, healthy and unpregnancy, 226±16g in body weight,3-month in age. Provided by experiment center of Hebei Medical University .After raised for 1 week, these rats were divided into 5 groups randomly, there were 10 rats in each group : Normal Control ,Model Group, Control Group, Low-dose Group and High-dose Group.Composition of decoction for warming meridian: Evodiae,Fructus 9g, Angelica 6g, Paeoniae radix 6g, Chuanxiong 6g, Ginseng 6g, Ramulns cinnamomi 6g, Donkey-hide glue 6g, Cortex moutan 6g, Zingiberis recens,rhizome 6g, Licorice root 6g, Pinelliae tuber 6g, Ophiopogonis tuber 9g. Buy from Shijiazhuang Rerentang. Use traditional decoction method, one dose par day, condense liquid apozem. High-dose group containing crude drug 1.3g each milliliter physic liquor (correspond 10times of clinical dosage), low-dose group containing crude drug 0.65g each milliliter physic liquor(correspond 5times of clinical dosage), maintain to reserve by placing 4℃Refrigerator .Normal control breed routinely, model group, low-dose group and high-dose group adopt method of freezing by ice water ,which putting rats in 0℃-1℃ice water 20 minutes, twice everyday, continuous two weeks. Control group lavages 100% liquid apozem 4ml per day, what fainting by aconite root and zingiberis,rhizome(1:1), continuous two weeks. Low-dose group and high-dose group lavages decoction for warming meridian , differencely to give 6.5g/kg/d,13g/kg/d(correspond 5 times and 10 times of clinical dosage)from the first day of making model ,once daily,2 weeks. Normal control lavages same voluminal distilled water. From the first day of making model, special messenger undertake vaginal smear oriented to time everyday, to observe the changes of estrous cycle of rats. After the last administration of making model, ambrosia but no prohibit water 10h,each animal swimming 5min as load, undertake vaginal smear every tow hours, select rats of diestrus to decapitate and to obtain blood, then use radio immunoassay assaying the content of serum corporin, aquadiol, andrusol, thylakentrin and luteinizing hormone.sterile operation to take head, immediately open the skull along midline, exposure cerebrum sufficiently and carefully to turn open, cut off chiasm opticum, separate and take out of hypothalamus by using nerves separate stick , take hypophyseal amicula carefully by using ophthalmology clamp ,then immediately wash repeatedly ,blot and weigh the hypothalamus and pituitary gland use twin distilled water .Open the abdominal cavity and select ovaries,weighed,4% glutaral and 1% osmic acid fixed, make organism to 10% homogenate used icecold separating medium,3000-4000rpm centrifuge 10min in low temperature, take supernatant low temperature ,high speed centrifugation 10min,the sedimentum is chondriosome. According to kit directions to determine the content of SDH. Make the segregative chondriosome to suspl using icecold homogenate, quassate chondriosome by ultrasonic disintegrator . According to kit directions to determine Na十-K十-ATPase and Ca2+-Mg2+-ATPase content.Result:1 General state of animalThe normal rats have clear and luster color pattern, fine spirit, clever active and reaction, normal meat and drink, urine and stool. After being frozen, the model rats appeared such symptoms as wriggly and few move , dull reaction, pale ear, claw and tail, like to heap, weak breathe, bloom color pattern, few the quantity of drinking water, clear rine, wet and rotten stool. Low-dose group and high-dose group rats only appeared slightly symptoms as chilly, like warm, few move .2 The changes of weight, drinking and eating of ratsThe rats in model group and control group body weight all loss, compared with normal control ,these were significance difference ( P<0.01 ) ,treated by decoction for warming meridian ,the weight of low-dose group and high-dose group all increase, compared with model group , these were significance difference ( P<0.01 , P<0.05 ) , compared with normal control ,these were no significance difference. The rats in model group and control group body weight all loss, but no significance difference.The rats in model group drinking decreased, but in control group were increased, compared with normal control, these were significance difference(P<0.01), treated by decoction for warming meridian , the rats drinking in low-dose group and high-dose group all increase, compared with model group , these were significance difference(P<0.01,P<0.05), compared with normal control, these were no significance difference. The rats in model group and control group eating less, compared with normal control, these were significance difference(P<0.01). Treated by decoction for warming meridian , low-dose group and high-dose group all increase, compared with model group , these were significance difference(P<0.01,P<0.05), compared with normal control, these were no significance difference.3 The changes of swimming time of ratsThe rats in model group and control group wimming time all decreased, compared with normal control ,these were significance difference(P<0.01) ,treated by decoction for warming meridian ,the swimming time of low-dose group and high-dose group all increase, compared with model group , these were significance difference(P<0.01,P<0.05), compared with normal control ,these were no significance difference. The rats in model group and control group wimming time all decreased, but these were no significance difference.4 The changes of estrous cycle of ratsThe estrous cycle lengthened in model group, mainly appeared longer in duration of anestrum, compared with normal control , these were significance difference(P<0.01), treated by decoction for warming meridian ,the estrous cycle and anestrum of low-dose group and high-dose group all decurtated, compared with model group , high-dose group have significance difference(P<0.01,P<0.05), low-dose group have no significance difference . Compared with normal control ,these were no significance difference. The rats in model group and control group estrous cycle and anestrum all decurtated, but these were no significance difference.5 The changes in ponderal index of ovary Ponderal index of ovary of rats in model group have decreased tendency, compared with normal control ,these were no significance difference(P﹥0.05) ,treated by decoction for warming meridian , ponderal index of ovary of rats in high-dose group have increased tendency, compared with model group, these were no significance difference(P﹥0.05) .6 The changes in gonadal hormone of serumCompared with normal control, gonadal hormone of serum of rats in model group decreased(P<0.01,P<0.05), treated by decoction for warming meridian , the content of E2,P,LH of low-dose group and high-dose group all increase, compared with model group , these were significance difference(P<0.01,P<0.05),the content of T,FSH of high-dose group increased, compared with model group , these were significance difference(P<0.01,P<0.05), the content of E2,P,LH,FSH of control group increased, compared with model group , these were significance differenc(eP<0.01,P<0.05), the content of E2,P of control group increased, compared with high-dose group, these were significance difference(P<0.05). 7The changes of Na+-K+-ATPase of hypothalamus, pituitary gland and ovariesCompared with normal control, the content of Na+-K+-ATPase of pituitary gland and ovaries in model group decreased(P<0.01,P<0.05), treated by decoction for warming meridian , the content of Na+-K+-ATPase of pituitary gland and ovaries of low-dose group and high-dose group all increased, compared with model group , these were significance difference(P<0.01,P<0.05), compared with normal control, these were no significance difference(P>0.05). The content of Na+-K+-ATPase of hypothalamus,pituitary gland and ovaries in control group increased, compared with model group , these were significance difference(P<0.01,P<0.05), compared with normal control, the content of Na+-K+-ATPase of hypothalamus in model group have decreased tendency, but no significance difference(P>0.05).8 The changes of Ca2+-Mg2+-ATPase of hypothalamus, pituitary gland and ovariesCompared with normal control, the content of Ca2+-Mg2+-ATPase of pituitary gland and ovaries in model group decreased(P<0.01,P<0.05), treated by decoction for warming meridian , the content of Ca2+-Mg2+-ATPase of pituitary gland and ovaries of low-dose group and high-dose group all increased, compared with model group , these were significance difference(P<0.01,P<0.05), compared with normal control, these were no significance difference(P>0.05). The content of Ca2+-Mg2+-ATPase of hypothalamus, pituitary gland and ovaries in control group increased, compared with model group , these were significance difference(P<0.01,P<0.05 ) , compared with normal control, the content of Ca2+-Mg2+-ATPase of hypothalamus in model group have decreased tendency, but no significance difference(P>0.05). 9 The changes of SDH of hypothalamus, pituitary gland and ovariesCompared with normal control, the content of SDH of pituitary gland and ovaries in model group decreased(P<0.01,P<0.05), treated by decoction for warming meridian , the content of SDH of pituitary gland and ovaries of low-dose group and high-dose group all increased, compared with model group , these were significance difference(P<0.01,P<0.05), compared with normal control, these were no significance difference(P>0.05). The content of SDH of hypothalamus, pituitary gland and ovaries in control group increased, compared with model group , these were significance difference(P<0.01,P<0.05), compared with normal control, the content of SDH of hypothalamus in model group have decreased tendency, but no significance difference(P>0.05).Conclusions:1 The changes of SDH and ATPase of hypothalamus, pituitary gland and ovaries have closed relationship with the happen of asthenia cold syndrome.2 The content of E2,P,T,LH,FSH of serum in rats of asthenia cold syndrome decreased, the content of SDH, Ca2+-Mg2+-ATPase ,Na+-K+-ATPase of hypothalamus ,pituitary gland and ovaries all decreased.3 Decoction for warming meridian can strikingly improve symptom of the model of asthenia cold syndrome ,regulate the estrous cycle and the level of serum E2,P,T,LH,FSH, regulate the content of SDH, Ca2+-Mg2+-ATPase ,Na+-K+-ATPase of hypothalamus, pituitary gland and ovaries,to make them normal. disproofed the changes of SDH and ATPase of hypothalamus, pituitary gland and ovaries have closed relationship with the happen of asthenia cold syndrome from treated point.