Preparation Of Doxorubicin-Tetrandrine Liposomes And Investigation Of In Vitro Cytotoxicity

Objective:Selecting doxorubicin and tetrandrine as model drug, preparation of complex liposomes and studying the method of preparation, evaluation the liposomes to reverse multidrug resistance of MCF-7 in vitro.Method: In this thesis, DOX-TET liposomes were prepared by (NH4)2SO4 -gradient method combined pH gradient method. The stability of DOX-TET liposomes was valuated with the leaking ratio, transmittancy changes,phospholipids content in 4, 25℃for 1 month. The MTT method was used to test cytotoxicity and reverse multidrug resistance of DL,D-T,DL-T,D-T-L on MCF-7/DOX.Result:Through orthogonal experimental design, one optimum recipes of DOX-TET liposome was founded that it showed DOX-TET /EPC 1:10, EPC/Ch 3:1, pH 7.6, incubation tempreture 50℃,concentration of (NH4)2SO4 was 250mmol/L. Under the optimal formulation, DOX and TET were encapsulated 90.77% and 80.12% respectively. The liposomes observed through transmission electron microscopy presents distribution of size uniformity ,the average diameter is about 90nm. The results of liposome MTT experiment suggested IC50 D-T-L