Extraction Separation Of Angelica Sinensis Polysacchride And It's Anti-Aging Effects In D-Galactose Induced Aged Rats

Objective: We have used a method that is different from traditional method to extracted ,isolated and gained an ASP from neutral angelica sinensis. ASP content was determined by the modified method of sulphuric acid-phenol. Different concentration ASP acted on subacute senile mouse model induced by D-galactose. We observed weight,behavior,brain and spleen section,spleen index,brain index and We measured SOD,MDA and GSH-PX content in the serum and brain tissue and cerebral cell apoptosis number to determine whether ASP has anti-aging effects and find its possible mechanism for further study of ASP pharmacology effects. The experimental study my be provide the new theory and experiment instruction to develop new anti-aging drug.Methods:①Extraction separation of ASP and content determining: We have used a method that is different from traditional method. We used ethanol to precipitate ASP, filtering, extracting in 80OC. We added saturable Ca(OH)2 solution to adjust PH to 10 and acetic acid to adjust PH to4-5. Solution was centrifugal filtered. After decoloring, filter liquor was decompress concentrated to 1/3 original volume. Alcohol was added into concentrate liquid, standing, filtering. Anhydrous ethanol,acetone,ether washed precipitate three times respectively. ASP was obtained when it was dried in 50 oC. ASP content was determined by the modified method of sulphuric acid-phenol;②Effect of ASP on anti-aging in D-galactose induced rats. Sixty mice were divided randomly into 6 groups (normal saline,D-galactose,lentinan,low,middle and high does of ASP groups). The D-galactose group,lentinan group and ASP groups were injected with 10% D-galactose 0.25ml/20g for 40 days. The ASP groups and lentinan group were given various dosage of ASP and lentinan 100mg/kg respectively by intragastric administration from the first day. We undergone diving platform test and Morris maze test to determine the study and memory ability . We observed the change on spleen index,brain index and weight. SOD activities,MDA content and GSH-PX activities were measured respectively in blood serum and brain tissue. The morphous of brain and spleen cells were observed with the method of HE and the apoptotic index of brain cells was determined with the method of TUNEL.Results:①ASP content results. ASP content was determined by the modified method of sulphuric acid-phenol in the 490nm wavelength. ASP content is 71.0%.②Effect of ASP on anti-aging in D-galactose induced aged rats. Comparing with model group, ASP could improve the study and memory ability of mice,increase the activities of SOD and GSH-PX and decrease MDA content in blood serum and brain tissue,markedly counteract the atrophy of the spleen,improve brain cell degeneration and decrease apoptotic index,and increase the number of lymphocyte.Conclution: The method that is different from traditional method to extracted ,isolated and gained an ASP is better than traditional method. We gained ASP in higher content with modified method and it will occur less effects on this experiment. ASP content was determined by the modified method of sulphuric acid-phenol accurately. This experiment suggests that ASP having good anti—aging action in mice. ASP may be developed as a drug that could prevent and treat senile disease.