Empirical Study After Application Of The Insulin In A Rat Model Of Traumatic Spinal Cord Injury

Objective: To observe neuroprotective effects of insulin on traumatic spinal cord injury in rat models and discuss its potential mechanisms.Methods: 60 SD rats were randomly divided into A group(control group) and B group(insulin group), with 30 rats in each group,The animal SCI model was established according to method Allen's, A group and B group were respectively infused with isotonic Na chloride or 1Iu/kg insulin through abdominal cavity , three times a day ,for 7 days. apoptosis neurons were observed on 8 hours, 3 days , 7 days, 14 days ,and 28 days after SCI ,and tigroid body, neurons ,GAP-43 positive cells,and BBB score were measured on 1day, 3 days , 7 days, 14 days ,and 28 days after SCI.Results: Neuron number ,GAP-43 positive cells,and BBB score in multitude time points were significantly higher in B group than in A group (p<0.05 or p<0.01). The number of apoptosis neurons in B group was less than in A group's(p<0.05 or p<0.01).Conclusions: Insulin has neuroprotective effects on traumatic spinal cord injury in rat models. It can promote recovery of function by depressing apoptosis of neurons and promoting recovery and regeneration of neuraxon.