Researches On The Changing Of BALB/c Mice Gastric Mucosa Mast Cell And Permeability Induced By Helicobacter Pylori

Objective: To investigate the role of Helicobacter pylori (Hp) in degranulation of gastric mucosa mast cell (MC) and permeability of gastric mucosa; therefore established the important role of Hp in the course of chronic urticaria (CU).Methods: 70 BALB/c healthy mice were randomized into 3 different groups. Hp group, consist of 30 mice, were given Hp liquid every 3 days for total 7 times; Control group was divided into 2, groups which was given alcohol 45% and BAS 2% seperatively with the same way as Hp group . Concentration of sucrose in the peripheral blood was used to assess the permeability of gastric mucosa by using high performance liquid chromatography (HPLC) before and after feeding. Toluidine blue staining of the gastric mucosa was also used to assess the total number of mast cells, the amount of degranulated mast cells and the percentage of degranulation after feeding.Results: There was significant statistical difference between the total number of mast cells (P<0.01) and the percentage of the degranulated mast cells (P<0.01) in those three groups, with the highest number and percentage were found in Hp group followed by alcohol group and BAS group. The concentration of blood sucrose after feeding was higher than before feeding both in the Hp and alcohol groups, with the higher difference found in the alcohol group; as for the BAS group, there was no significant statistical difference (P>0.01), but compared to the alcohol group and BAS group, there was significant difference (P<0.01).Conclusions: Hp infection increases the number of gastric mucosa mast cells, and increases degranulation which releasing histamine and a number of vasoactives mediator to peripheral blood hence causing vasodilatation of the gastric mucosa, wheal and other typical symptoms of urticaria. Hp infection can increase the permeability of gastric mucosa , therefore increasing the absorption of antigen, anaphylactogen and other inflammatory factors through the impaired mucosal barrier; and induces or worses chronic urticaria.