The Treatment Effect Of Ultrasonic Microbubble Contrast Agent And Focused Ultrasound On Rat Glioma

Objective: The glioma nude mice animal models are set as a research platform. Through the tail vein injecting microbubble ultrasound contrast agent in the nude mice and irradiated by high intensity focused ultrasound (HIFU) on tumor tissue, that is uesd to study the changes of proliferating cell nuclear antigen in glioma cells and their apoptosis characteristics in order to test the development of glioma cells after HIFU ablation. It mainly focuses the discussion of role about the treatment of HIFU combined with ultrasound contrast agent microbubbles on glioma nude mice.It provides a theoretical basis for HIFU applying in the adjuvant treatment of glioma clinical.Methods: Wang Hai-very-ultrasound therapy was adopted as an assemble energy experiment of HIFU. The output power was 5W and adopted the corresponding optimal configuration. The irradiation time was from 30 S, 40S, 50S to 60S respectively. 24 nude mice with subcutaneous glioma were divided into the experimental group (n = 12) and control group (n = 12). In the experimental group, the mice were injected ultrasound contrast agent microbubbles by the tail vein and irradiated under HIFU. Set the time immediately , 3 days and 5 days after HIFU ablation in which two nude mice are sacrificed, made to 5 micrometer paraffin section and put under the HE staining-histological for observation. Proliferating cell nuclear antigen and the apoptosis of the glioma cells with 3-day and 5-day were studied to analyze Proliferation and apoptosis changes after HIFU ablation within targeted region.Results: under the same of instrument output parameters of Wang Hai Star ultrasonic radiation therapy, the various killing effect degrees of HIFU on glioma cells were observed in the experimental group and the control group:â‘ In the control group, proliferating cell nuclear antigen and apoptosis degree was significant difference in 30 S, 40S and 50S (P <0.01), and there was no significant difference in 50 S and 60 S (P> 0.05);â‘¡In experimental group, proliferating cell nuclear antigen and apoptosis degree were significant differences in 30 S and 40 S (P <0.01), and there was no significant difference in 40s,50 S and 60 S (P> 0.05);â‘¢proliferating cell nuclear antigen and apoptosis degree in 40S in experimental group and 50S in control group were no significant difference (P> 0.05), and were significant difference in 40S in experimental group and 40S in control group(P <0.01)Conclusion: under the circumstances of constant output power and irradiation time, the HIFU treatment for the glioma cell killing effects and the length diaplays a positive correlation. Beyond the scope of a certain period of time, extending the irradiation time of HIFU on glioma tissues does not enhance the killing effects of HIFU on glioma cells; microbubble ultrasound contrast agent can not only enhance the treatment of HIFU on rat glioma, but also reduce the HIFU irradiation time and decrease the tissue injury on the surrounding.