Studies On Chemical Constituents Of Tibetan Medicine Suba And Of Their Application

Suba is the transliteration of Tibetan.It is a traditional Tibetan medicine.The medicinal materials come from the dry roots of Silene aprica Turcz.It can be used as a diuretic,and it can improve hearing and vision.The chromatographic conditions of HPLC for Suba have been investigated in this article.The optimal chromatographic condition is DIKMA Kromasil C₁₈ column(4.6×200mm,5μm)and acetonitrile(A)—0.4%phosphoric acid(B)as mobile phase at a flow rate of 1mL/min with sample size 10μL using gradient elution method;The mobile phase variate with time as follows,0～10min(54%～53%A), 10～25min(53%～52%A),25min(52%A);The detective wave length was 220 nm.and the column temperature is 30℃.The HPLC map was excellent and the testing time was short.The number of the peak is large relatively,and the spaces between peaks are proper.It lays the groundwork for the rapid separation of contents of Suba,the establishment of HPLC fingerprint and the standarationg of Suba medicine materials.The medical materials were extracted by methanol,and the extractions were separated by 0.5%Na₂HPO₄ Silica gel column chromatography.4 monomeric compounds were isolated,and their structures were elucidated by physicochemical determinition,spectroscopic analysis and consultation of documents.All of them are saponins.They were 3-O-β-D-glucuronopyranosyl oleanolic acid-28-O-β-D-glu-copyranoside; 3-O-β-D-glucopyranosyl(1→2)-β-D-glucuronopyranosyl oleanolicacid-28-O-β-D-glucopyranoside;4-carboxyl ursolic acid-28-O-β-D-glucopyranoside; 19-hydroxyl ursolic acid-3-O-a-L-rhamnopyranosyl(1→2) -β-D-glucuronopyranosyl(1→2)-β-D-xylopyranoside.This thesis investgated the exploration of Suba preliminarily as well.Suba natural detergent was maded.The product was a colorless uniform viscous liquid with stable performance.Compared with synthetic detergents,not only the same scrubbing effect it has,but also many advantages were found,such as low toxicity, easy degradation,and environment friendly,et al.So it has great potential for development.