Effects Of High Frequency Oscillatory Ventilation With Perfluorocarbon On Breath And Influence On Level Of Interleukin-10(IL-10) And Interleukin-18 (IL-18) In Rabbits With Acute Respiratory Failure

Objective To investigate the effects of high frequency oscillation ventilation(HFOV) with perfluorocarbon(PFC) on breath and influence on level of interleukin-10(IL-10) and interleukin-18 (IL-18) in rabbits with acute respiratory failure induced by water vapour inhalation injury.Methods Twenty four New Zealand rabbits with acute respiratory failure induced by water vapour inhalation injury were randomly assigned to three groups: conventional mechanical ventilation (CMV) group , high frequency oscillation ventilation (HFOV) group and HFOV+ PFC group. All rabbits were ventilated for four hours ,Blood gases were measured at 1,2,3,4 hour during experiment, 4 hours later got blood and bronchoalveolar lavage fluid (BALF) to measure the level of interleukin-10(IL-10) and interleukin-18 (IL-18) with method of ELISA。Observe the lung and got the part of the middle lobe of right lung for histological observation.Results After acute respiratory failure (ARF) established PaO2 in the three groups decreased below 60 mmHg ( P <0.01) ,and intergroup differences were not found( P > 0.05). PaCO2 in the three groups increased and PH decreased( P<0.01), and intergroup differences were not found( P > 0.05). PaO2 in the three treatment groups had increased at one hour after treatment, HFOV+ PFC group was higher than HFOV group( P < 0.01 ), HFOV group was higher than CMV group( P < 0.01 ).After four hours, PaO2 of three groups increased, HFOV+ PFC group was higher than HFOV group at the same time( P < 0.01 ), HFOV group was higher than CMV group at the same time( P < 0.01 ). After four hours, differences of PaCO2 and PH were not found( P > 0.05).level of IL-10 in blood and BALF: HFOV+ PFC group was higher than HFOV group( P < 0.01 ), HFOV group was higher than CMV group( P < 0.01 ); level of IL-18 in blood and BALF: CMV group was higher than HFOV group( P < 0.01 ), HFOV group was higher than HFOV+ PFC group ( P < 0.01 ). Histological injury score was lowest in HFOV + PFC group and highest in CMV group.Conclusion HFOV with PFC can better improve arterial oxygenation in less time, and increase IL-10, decrease IL-18 ,alleviate pulmonary edema and injury, which may be a optimal method for the treatment of rabbits with acute respiratory failure caused by water vapour inhalation injury.