Study On Effect Of Biocatalysis On The Extraction Of Aconitum Alkaloids From Prepared CaoWu

Along with the petrochemical resources and the energy crisis, environment and the society crisis become worse increasingly, as the important method for develpoment of the green chemistry and green chemical engineering, the development of the biocatalysis will have significant effect on medicine, agriculture, food, life science and so on. It is a new technology to extract and separate active constituents from natural plants with enzyme catalysis. Cell walls in plants hinder the extraction of effective constituents. The cell walls will be decomposed when proper enzymes are introducted to act on natural plants, which is profitably to the extraction of effective components.Cellulase was used to pretreat prepared CaoWu and then aconitum alkaloids were extracted by alcohol in this paper. The optimum catalysis conditions of cellulase and its effect on yield rate of aconitum alkaloids were studied. The yield rate and purity by cellulase catalysis method was compared with others. Meanwhile, the effect cellulase catalysis on conventional alcohol extraction and the mechanism of cellulase catalysis were also researched here.The optimum conditions that cellulase catalyzed and transformed aconitum alkaloids were at 50℃, pH 4.5, dosage of cellulase 1.0% ( 0.01 g cellulase in 1 g material) and the reaction time of cellulase 4 h. Compared with the 4 other methods, the yield rates of aconitine and hypaconitine were much bigger. It indicated that the hydration and degradation on cell wall of prepared CaoWu enhanced permeation of cell wall and minished mass transfer resistance. It made aconitum alkaloids be taken out easier. The yield rate of aconitum alkaloids was improved obviously after the prepared CaoWu was pretreated by cellulase. The yield rates of aconitine and hypaconitine were improved from 0.00149% to 0.00235% and 0.0233% to 0.0386%, and rised 57.9% and 66.0% respectively. The yield rate of aconitum alkaloids was improved from 0.0475% to 0.0820%, and rised 72.6%.The content of aconitine and hypaconitine in product by cellulase catalysis was 48.9% according to HPLC chromatogram, and the purity of aconitum alkaloids was 97.9% according to UV. And the yield rate of aconitum alkaloids was 0.0820%. This paper shows that cellulase catalysis technology will play a broad part in the extraction of active constituent from natural plants.