Effect Of Curcumine On The Expression Of Matrix Metalloproteinase-9 Induced By Tumor Necrosis Factor A In Human Umbilical Vein Endothelial Cell

Background and objective of researchCardiovascular disease has become one of hackneyed diseases threaten human health ever since a long time ago . and artherosclerosis threaten human health and life severity , incidence and death rate of coronary atherosclerotic herat disease caused by artherosclerosis hold the first place of cardiovascular disease , and have ascensus tendency , further more make more youthful gradually , this question evoked more and more people pay close attention to it generally .Recently the investigation shows that the metalloprotease is one of the consequence factor to cause the plaque astable , they can by degrdn collagen fibers and connective tissue in desmohood to make the desmohood thinningz , thereby emerge astable plaque . metalloprotease-9 also called gelatinase B , is one important members of metalloproteases family , its effect substrates include gelatinaseⅣ,V-type gelatinase collagen,stress protein and so on . And V-type gelatinase collagen is the important ingredient of atheromatous plaque basal lamina and desmohood , so metalloprotease-9 make an important effection in the extracellular matrix degrdn process of atherosclerotic plaque , so the research of metalloprotease-9 will open a new prospect for arteriosclerotic diagnose,therapy and prophylaxis .My experiment by investigating the effect of curcumine on the expression of matrix metalloproteinase-9 in human umbilical vein endothelial cell , and investigating the effect of curcumine on the expression of matrix metalloproteinase-9 induced by Tumor Necrosis Factorαin human umbilical vein endothelial cell . To investigate the pharmacologic action of curcumine futher , and to provide dependable experiment accordings for clinical application of curcumine . Providing new thinking ways and method for artherosclerosis acrmegalic treatment and prophylaxis on clinical .Investigate methods and content1. culture archaegenerate human umbilical vein endothelial cell in vitro , and appreciate it by FⅧfactor , then passage the HUVEC.2. The effect of curcumine on the expression of matrix metalloproteinase-9 in human umbilical vein endothelial cell .Divide the grew well human umbilical vein endothelial cell which after passaged into six groups ( which is blank group,curcumine density 6.25μg/ml group,curcumine density 12.5μg/ml group,curcumine density 25μg/ml group,curcumine density 50μg/ml group and curcumine density 6.25μg/ml group ), to add different density of curcumine to treat the HUVEC for 24 hours , and determine the expression of matrix metalloproteinase-9 by cytochemical staining and Elisa . Express the result by the form of x±S ,the compare the differents between groups by analysis of variance .3. The effect of curcumine on the expression of matrix metalloproteinase-9 induced by Tumor Necrosis Factorαin human umbilical vein endothelial cell .Divide the grew well human umbilical vein endothelial cell which after passaged into six groups ( which is blank group,curcumine density 6.25μg/ml group,curcumine density 12.5μg/ml group and curcumine density 25μg/ml group ) , to add different density of curcumine to treat the hUVEC for 24 hours , after induced by same density of TNF-αfor half an hour , to determine the expression of matrix metalloproteinase-9 by cytochemical staining and Elisa . Express the result by the form of x±S ,the compare the differents between groups by analysis of variance .Results1. The human umbilical vein endothelial cell can express metalloproteinase-9 under the normal fundament condition , once the human umbilical vein endothelial cell culture in vitro emerge the monolayer of pebble , then they can express the matrix metalloproteinase-9 .2. The investigate result shows that the expression of matrix metalloproteinase-9 obviously decreased after the human umbilical vein endothelial cell effected by curcumine , and the expression density decreased by the accrescence of curcumine effect density , there is obviously contrast between groups ( P<0.01 ) .3. The investigate result shows that the human umbilical vein endothelial cell can express matrix metalloproteinase-9 under the normal fundament condition , the matrix metalloproteinase-9 expression density is accrescenced after induced by Tumor Necrosis Factorα, the expression of matrix metalloproteinase-9 obviously decreased after the human umbilical vein endothelial cell effected by curcumine , and the expression density decreased by the accrescence of curcumine effect density , there is obviously contrast between groups ( P<0.01 ) .Discussion1. The human umbilical vein endothelial cell culture in vitro can express matrix metalloproteinase under the normal fundament condition , after effected by curcumine , the masculine expression of matrix metalloproteinase-9 obviously be inhibited , and the expression density decreased by the accrescence of curcumine effect density .2. The matrix metalloproteinase-9 masculine expression density is accrescenced after induced by Tumor Necrosis Factorαof human umbilical vein endothelial cell culture in vitro , an the expression density of matrix metalloproteinase-9 obviously decreased after the human umbilical vein endothelial cell effected by curcumine , and the expression density decreased by the accrescence of curcumine effect density .3. Curcumine can inhibited the matrix metalloproteinase-9 masculine expression of human umbilical vein endothelial cell culture in vitro , for decreasing the reconstitution of extracellular matrix , strengthening the stability of atherosclerotic plaque , recovering the function of damaged blood vessel endothelium ,preventing the emerge of thromb , blocking up the proinflammatory factors and degrading the probability of arterial caliber shink or obliterate , play an important part in preventing and curing the nosogenesis and growth of coronary artery disease .