Effect Of Rosmarinus Officinalis Extract On Senescence And Acute Liver Injury In Mice

Senescence relates to human`s lifespan and death Closely, which is a complex physical process. About mechnisms of senescence, many theories have been promulgaetd. Among all the theories of aging, demonstrated by more and more researches,free radical theory is considered as the most believable theory. Free radical theory of aging predicts crucial role for free radicals produced by external factors and pathological disorders in the initiation of aging,which is based on the hypothesis that react with biological molecules,increase DNA damage,result in bond breakages,accelerate lipid peroxidation on biological membranes,and accumulations of reacting products influence the ability of tissues and organs to carry out critical biological functions.Based on the free radicals theory of aging, current researches have revealed potential antiaging effect of foods with antioxidant properties. Emerged as a novel functional food additive, Rosmarinus officinalis extract has been of particular interest in recent years. It is an efficient antioxidant and singlet oxygen quencher. Rosmarinus officinalis extract has been shown to be associated with decreased risk of chronic diseases such as cancer and cardiovascular diseases, as well as enhanced immune function in numerous studies.Thus, on the basis of previous investigations, this study was designed to evaluate antisenescence effect of Rosmarinus officinalis extract from several aspects: behavior status, antioxidant system (SOD, MDA) and hypoxia tolerance ability of mimetic aging induced by D-galactose. We got the conClusions that Rosmarinus officinalis extract could increase serum and brain SOD and reduce serum and brain MAD, increase and time of anti-hypoxia. In conClusion, Rosmarinus officinalis extract in a dose of 4g/kg can prolong drosophila melanogasters lifespan, improved life quality of aging mice and suggested significant antisenescence potential in this experiment.Moreover, the effects and mechanisms of Rosmarinus officinalis extract and rosmarinic acid by oral administration on carbon tetrachloride-induced liver injury in mice were also examined. Liver is the largest substantial gland and central station of metabolism in vivo.Being the important detoxication organ,the liver is the important target of drugs,xenobiotics and oxidative toxicant.Liver injury is the starting link and common approach of serious liver disease occurrence,development and finally moving towards the liver function failure.The liver injury being caused by various harmful factors were manifested as hepatic necrosis,fatty liver,cholestasis,liver fibrosis,liver cirrhosis and liver cancer and so on.In order to clarify the involved details, we investigate the effects of Rosmarinus officinalis extract and rosmarinic acid on chemical liver injury in vivo in the present study. Then, alanine minotransferase (ALT), asparatate aminotransferase (AST), trigly -ceride (TG) in serum were performed in the following in vivo experiments. Subsequently,the hematoxylin eosin(HE) pathological section were performed to clarify the possible mechanisms.Taken together,the results indicated:Rosmarinus officinalis extract in a dose of 200 mg/kg and rosmarinic acid in a dose of 20mg/kg significantly suppressed the elevation of ALT, AST and TG in blood serum. They had obviously protective effects on acute liver injury induced by CCl4 in mice which might be related the effect of anti-oxidation, protection of liver cell membrane and clear free radical.