The Effects Of StrongerNeo-MinophagenC-liposome On Proliferation Of Human Hepatic Stellate Cells

Objective To evaluate the effects of StrongerNeo-MinophagenC(SNMC) -liposome on the proliferation of human hepatic stellate cells, and explore a new kind of pharmaceutical dosage form on the treatment of hepatic fibrosis.Methods 1.SNMC-liposome was made by ultrasound microfilm method.2.HSCs were incubated in medium when they during the exponential phase of growth with different concentrations of SNMC and SNMC-liposome(0.03125,0.0625 mg/ml,0.125 mg/ml and 0.25mg/ml respectively) or without SNMC and SNMC-liposome for 24 hours. The proliferative inhibition rate of the HSCs was determined by MTT assay.3.The conformation of HSCs was observed by microscopy after Giemsa staining. High resolution pathological image analysis system was used to determine the areas of cells in every visual field under microscope. Results 1. The proliferative inhibition rates of the HSCs induced with different concentrations of SNMC and SNMC-liposome for 24 hours determined by MTT assay was significantly exceed compared with that of the other two groups. And the proliferative inhibition rates of the SNMC-liposome group was higher than the SNMC group with the same concentration (P<0.05). But the pure HSC control group had no difference with the liposome group (p>0.05). The proliferative inhibition rates of HSCs treated by SNMC and SNMC-liposome increased concentration-dependently.2. The areas percentage of cells with Giemsa staining in every visual field under microscope decreased concentration-dependently. And the proliferative inhibition rates of the groups treated by SNMC-liposome is lower than the groups treated by SNMC when they are in the same concentration. And there was no difference between pure HSC control group and liposome group. Same result was indicated by the calculating the areas of cells in every visual field under microscope.Conclusion SNMC and SNMC-liposome can inhibit the proliferation of activated HSCs incubated in vitro. And we also proof that the effect of inhibition can strengthen after SNMC encapsuled by liposome. The liposome has no effect on the activated HSCs in vitro.