Effect Of Deferoxamine With Homoharringtonine On Apoptosis And Cell Cycle Of K562 Cell In Vitro

Objective:To study the effects of Deferoxamine(DFO) with Homoharringtonine (HHT) for human chronic myelocytic leukemia cell line(K562) apoptosis and cell cycle in vitro.Methods:The effects of DFO,HHT alone and both for the proliferation of K562 cells were analyzed by MTT reductionassay,and morphologic changes of apoptosis by HE and Annexin V-FITC stains,cell apoptosis rate and cell cycle by flow cytometry in vitro.Results:â‘ MTT reduction assay revealed that the inhibition of DFO to the proliferation of K562 cells was in a does- and time-dependent manner.â‘¡DFO with HHT showed significant synergistic anti-tumor effects for K562 cells.50,100,200μmol/LDFO and 0.5μg/ml HHT showed synergistic by Kim's formula.â‘¢By using flow cytometry the hypodiploid peak was observed in culture with DFO cells.The apoptosis rate in a does-and time-dependent manner and of DFO combined with HHT were obviously higher effect than that of anti-tumor drugs alone.â‘£K562 cells were blocked in G₀/G₁ phase in a dose dependent manner.The highest G₀/G₁peak was detected at the time of after 36h and DFO combined with HHT were obvious blocking cell cycle.Conclusion:â‘ DFO could inhibit the of K562 cells obviously in a doesand time-dependent manner.â‘¡DFO could induce K562 cell apoptosis and block K562 cells in G₀/G₁ phases.It is concluded that the induced cell cycle arrests may be an important mechanism of DFO on anti-tumor effects.â‘¢DFO combined with HHT displayed not only inhibitory effects but also inducing apoptosis effects on K562 cells.