The Empirical Research Of Mailuoning Compound In The Treatment Of Hormone-ANFH Mechanism

1 Background:Avascular necrosis(Avascular Necrosis of the Femoral Head SR ANFH) is the result of trauma or injury,and many other reasons for avascular necrosis of the bone cells of femoral head,bone marrow,blood cells and fat cells,bone nutrition interruption,finally result in Increase or reduce the density of the femoral head,bone cystic changes,bone fracture,the femoral head collapse deformity and a series of diseases,known as femoral head avascular necrosis.Because of bone necrosis is not caused by bacterial infection,also known as axenic avascular necrosis of the femoral head.It is the orthopedic common diseases,due to a variety of causes destructing the blood supply of the femoral head,the active component of bone (including bone cells,bone marrow Cell and fat cell) death caused by a pathological process.As the incidence reasons are complicated,leading to lesions of cartilage damage, osteonecrosis of the femoral head of change,lose joint function and has a high morbidity.Avascular necrosis in the incidence of middle-aged mainly 30 to 40-year-old Green,40-80%of the patients for bilateral disease,according to their natural illness course of the study showed that about 80 percent of untreated ANFH Femoral head collapse will occur during the 1-3years.Once the femoral head collapse,there will be varying degrees of pain and dysfunction,serious patient had to accept artificial hip replacement,It has greatly affected the quality of patients life.Necrosis of the femoral head happens not only for the basis of systemic factors,but also incidence of local particularity,the body repair its own existence after Osteonecrosis, performance on mesenchymal cells on live bone and capillary proliferation invasive necrosis of bone and replace marrow,undifferentiated mesenchymal cells attached to the surface necrosis bone differentiation into skeletogenous cell and form new bone.The strategy and the goal on the treatment of this disease on should be active in the treatment of the original disease, improve the general state of imbalance at the same time,partial lifting of insufficient blood supply,improve microcirculation and reduce vein Stagnation and reduce pressure within the bone to prevent adverse biological stress factors influence and take appropriate Functional training,to stop or reverse pathological process,induction and promotion new bone formation and reconstruction,the femoral head to repair and restore the hip joint function,in Chinese medicine ANFH occurs mainly because of bad blood disease internal resistance,on the one hand block Weiqi through the muscle and skin their health,loss of protected function:On the other hand block of qi and blood running in local,caused partial loss support,and lead to invasion evil easily,evil blood and invasion evil maxed for the Bone Bi.As《Lingshu.Thief wind》said:If the falling something,the worsen blood will store.For the current therapy to osteonecrosis of the femoral head mainly depend on the surgery in china,the recent results is clearly,but not an ideal long-term effect.Chinese medicine combination therapy reserve bone structure and function proceeding from the whole concept focus on both internal and external,ax activity and still,bones and tendons take with the principle of a Differential Treatment,made complete deficiency surgical therapy.My teacher accroding on the basis of traditional Chinese medicine theory and clinical practice,created a Mailuoning composite liquid therapy avascular necrosis by nerve block, and achieved satisfactory results.Mailuoning composite liquid make uo with Mailouning,2% lidocaine and 5%sodium bicarbonate by a certain percentage from compatibility.Mailouning is pure Chinese medicines combine with Achyranthes,Scrophulariaceae,honeysuckle, Dendrobium composition,with function of Promoting Blood Circulation and Relieving Blood Stasis,nourishing yin and blood,Qingrejiedu,Mainly use for the treatment of thrombosis obliterans,and sequelae of cerebral thrombosis and other diseases.There is no moving blood medicine in Mailuoning composit but it has active ingredients for chemical chlorogenic acid, Azerbaijan dental materials,and the fat within the foreshore Artemisia coumarin compounds, to expand blood vessels,improve microcirculation,Improve fibrinolytic activity,reduce fibrinogen levels,lower blood viscosity,increasing blood flow,with an anticoagulant, thrombolytic effect.it prove that have the effect of promoting blood circulation,lidocaine can cut off the vicious cycle of pain by Local nerve block,lift muscle cramps and lower the pressure within the hip joint,while lidocaine block femoral artery near the sympathetic, diastolic blood vessels,increase blood supply of the femoral head around.This method really understands the disease from Chinese medicine blood stasis,and start with improving the local blood supply of femoral head actively,This study's main purpose is observing the effect of Mailuoning compound in the treatment of hormone-ANFH mechanism by nerve block. theoretical basis for clinical treatment.To this end,the topics prepare to de research in three areas from the morphology,immunohistochemistry,blood lipid changes.2 Purpose of the studyThrough the application of ANFH model in Long-term low-dose hormone of Mailuoning composite therapy to intervene observing before and after treatment changes.Application HE staining methods,observed ANFH the pathological changes from the morphology in the application of immunohistochemistry methods to observe the rats' in the femoral head impact on bone growth and repair of a variety of growth factor's in the changes.Mailuoning test compound in the treatment of hormone-necrosis of the femoral head,and further understanding Mailuoning compound in the treatment of the disease mechanism from morphology and molecular biology,to provide a theoretical basis for clinical treatment.3 Research Methods3.1 Materials and MethodsSelection of 26 adult male Wistar rats(second degree by the China Institute of Traditional Chinese Medicine Centre for laboratory animals,animal qualified for the No.SCXK(Army) 2002-001) weight 290±20g,Select 20 copies of the hormone necrosis of the femoral head animal model:intraperitoneal injection of acetic acid prednisolone 6.7mg/kg,3 times/ week,for three weeks,after the completion of the model group were randomly divided into hormone(for short hormone group),10,Mailuoning compound in the treatment group(for short the treatment group) 10,and the remaining six is the blank group(for short blank group).The treatment group,hormone group and blank group were taken the rat model in the first day after nine weeks.with 0.3 percent sodium pentobarbital intraperitoneal injection anesthesia(1ml/100g),paunch and take abdominal aorta blood 5 ml,fasting 12 h before Acquisition,were put in the tube apartly.4℃centrifugal 3500 rpm,15 minutes,separation of serum,4℃refrigerator preservation,sent to the WanNing hospital biochemical laboratory tests total cholesterol,triglycerides.At the same time to remove the paraffin sections of the femoral head,HE stain,Observed the femoral head of the pathological changes in organizational structure by light microscopy and,through image analysis instrument analysis HE staining biopsy rate of lacunae,bone lacunae area,Trabecular bone area of quantitative analysis for vascular endothelial growth factor(Vessel endothelial cell growth factor,VEGF),bone protein(Bone morphogenetic proteins,BMPs),transforming growth factorβ1(Transforming growth factor-β1,TGF-β1),Ⅷ-related antigen(Ⅷfactor fos-related antigen,Ⅷ-R Ag) Immunohistochemical staining SP method,using image analysis instrument analysis VEGF,BMPs,TGF-β1,the positive area ratio,the number of positive cells,Ⅷ-RAg of the MVD.3.2 statistical methodsSPSS13.0 software used for statistical analysis,the measurement data group compared the single-factor analysis of variance,the measurement data to mean±standard deviation(x±S) said,P＜0.05 for statistical significance.4 Conclusion(1).A simple small dose of the hormone long-term approach can be successful establishment of hormone-ANFH model,to better clinical response the pathological process of the hormone ANFH.(2).Necrosis of the femoral head of hormones in hormone-induced fat metabolic disorders in the bone-related gene expression changes were related.(3).Through Mailuoning compound treatment can microvascular and growth factor generate expression closely related to bone cells growth increased and promote bone repair,then cure hormone osteonecrosis of the femoral head.(4).Mailuoning compound used in the treatment can improve by high blood lipid situation caused the hormone and restore balance the body lipid metabolism and reduce fat accumulation in the marrow,effectively reduce the pressure within the bone,increase the blood supply of necrosis.(5).Mailuoning injection has functions of Promoting Blood Circulation and Relieving Blood Stasis,nourishing yin and blood,Qingrejiedu.The experimental results show that Mailuoning injection with lidocaine,sodium bicarbonate according a certain percentage with a nerve block approach in the treatment of hormone necrosis of the femoral head,is effective through increased and microvascular and the growth factor of production which closely related bone cells generate,and to improve high blood lipid status caused by the hormone,It is the main mechanism of that therapy.