The Preliminary Experimental Study On Reparing The Cartilage Defect In Rabbit Knee Joint By Tiuuse-engineered Collagen Type Ⅱ Sponge And Its Derivative Product

Objective:by the means of producing animal model of articular cartilage injury and producing tissue-engineered collagen typeⅡsponge and its derivative product to observe the growing conditions of the cartilage cells in injury animal model which accepted remedial interventions by collagen typeⅡsponge and its derivative product,and to preliminarilily research the efficacy of collegan typeⅡsponge and its derivative product in curing the articular cartilage defect,to compare the performance of the different two kinds of material,two different techniques,and to derive experiences about animal experiment,and elaborations of biomaterial.Methods:(1)to abstract and purify collagen typeⅡby improved techniques;(2)to produce collagen typeⅡsponge and collagen typeⅡ- hyaluronic acid composited biomembrane by two different techniques;(3)to evaluate the physical-chemical proformance,biocompatibility,mechanical property and porosity of the two kinds of mentioned biomatieral;(4)to design a scientific injury animal model by taking advantage of formerly practical experiences.(5)to carry out the animal experiment by means of randomization,grouping and comparison.(6)by the means of MRI, histological observation,etc,to evaluate the repairing effect of the rabbit knee joint cartilage cells which accepted a remedial intervention by the different two kinds of biomaterial.Result:the collagen typeⅡsponge produced by improved technique has satisfied biocompatibility,and have an advantage over nowadays predominant products in physical-chemical proformance such as purity.Biomechanics results of the comparison between the two kinds of material show collagen typeⅡsponge has less modulus of elasticity,better expansivity and plasticity so as to be more apt to be fixed into defect in vivo as bio-matrixes.Image analysis of Collagen Spontaneous Fluorescent Delamination Scaning shows a mean pore diameter of 175.20±50.25μm~2 collagen of typeⅡsponge,which gets more close to optimal pore diameter than that of collagen typeⅡsponge- hyaluronic acid composite biomembrane.By a transversal comparison of various observational result such as MRI,HE of the same time point, less inflammatory reaction and faster inflammatory fluidify absorption than the other two groups,and initial neogenesis cartilage signal are found in group-collagen typeⅡ,and the results of Safranin O,immunology-histology chemical prove that the neogenesis cartilage to some extent has abilities of secreting glycosaminoglycan and ground substance of collagen typeⅡ,which shows that collagen typeⅡsponge has not only obvious efficacies of curing cartilage but restriction against inflammatory reactions to some extent,while group- CⅡ+HA have adverse effects,and blank comparison group have effects between the other two groups.Synthetize evaluations show collagen typeⅡsponge has an obvious efficacy in curing cartilage defect while the producing teniques of collagen typeⅡsponge- hyaluronic acid composite biomembrane need to be improved.Conelusion:1.The collagen typeⅡderived from improved techniques has satisfied physical-chemical performance.2.The collagen typeⅡsponge has satisfied efficacy on curing rabbit cartilage defection,which underlies further cell culture tissue-engineering reseach.3.Mixing collagen typeⅡand hyaluronic acid directly will lead to adverse interreaction,and somehow weaken the efficacy in curing cartilage defection.So,the elaboration technique of producing collagen typeⅡ- hyaluronic acid composited biomembrane require to be improved. 4.By combining MRI with pathohistology and immunology methods,we could observe the result of cartilage curing more effectly and roundly.