Effect Of Bifidobacteria On Intestinal Bacterial Translocation In Experimental Obstructive Jaundice

Objective : To investigate the effects of enteral administration of bifidobacteria on intestinal bacteria translocation that is induced by obstructive jaundice in rats.Methods: A total of 30 Wistar rats were randomly divided into three groups of 10 each:Ⅰ, sham operated;Ⅱ, bile duct ligation (BDL);Ⅲ, BDL + bifidobacteria treatment group. GroupⅠ:10 rats underwent laparotomy, common bile duct (CBD) was not actually ligated and transected, but sham ligation of CBD was performed. GroupsⅡ,Ⅲ:20 rats underwent laparotomy, CBD ligation and transection. Terminal ileal samples for histology, tissue specimens from the mesenteric lymph nodes (MLNs), spleen (SPL), and liver (LIV) for culture, and portal blood samples for the liver function and endotoxin measurements were obtained 10 days later.Results: Compared to the controls, a significant increase in contaminated MLN samples was noted in groupⅡ(p < 0.05) but were significantly reduced in groupⅢ(p < 0.05). The liver function was significantly improved in groupⅢthan in groupⅡ. The value of endotoxin in group III was significantly lower than that in group 0282 { (0.74±0.20 )EU/ ml vs(1.99±0.31)EU/ ml , p < 0.01 } . Histologically , Shortened and blunted villi, and even subtotal villous atrophy were observed in the small intestinal mueosa in BDL group. However, the damaged ileum mucosa was markedly repaired after bifidobacteria-supplemented formula.Conclusions: Enteral administration of the probiotic bifidobacteria in the presence of obstructive jaundice improves the ileal histology, ameliorates BT and endotoxemia.