Pituitary Adenoma Studied By Proteomics

Partâ… The different protein expression in human pituitary adenoma tissue studied by proteomicsObjective Analyze different expression profile proteomics between pituitary adenoma tissue and nomal pituitary tissue by 2-DE combind with MALDI-TOF-MS,to find possible clew about the pathogenesis of pituitary adenoma,and to provid clew and foundation for clinical application about diagnosis markers and drug target.Methods Collect 5 samples in each type of PRL,GH and no function pituitary adenoma tissue and 3 samples of normal pituitary tissue from neurosurgery department of huashan hospital in the period 2006.9 to 2007.9,all samples had comfirmed by pathology. Then analyze composite sample by proteomics with follow steps: extraction total protein of each samples,2-DE,fast silver staining, choose and quality control of different protein dots,gel incise and protein enzymolysis,MALDI-TOF-MS.Finally we analyze the different protein expression profile between pituitary adenoma and normal pituitary.Result Establish 2-DE atlas of each type of pituitary adenoma and normal pituitary,which in good resolution and reproducibility.it reveals that most protein dots are in the range of MW 15-75kDa and PI 4-8,these hint the homology between pituitary adenoma and normal pituitary. After soft analysis and quality control,we confirm utility different protein dots as follow:PRL 111,GH 70,no function 75. The all utility different protein dots in the 12 atlas analyze by MALDI-TOF-MS,endo-rectify,index in MASCOT database and bioinformatics analysis,then identify 35,21,24 protein respectively,including SOD1,CA1,ESD,PRL,GH.Conclusion Establish 2-DE atlas of pituitary adenoma tissue and normal pituitary tissue Successfully. Discover CA1,ESD,PGK1,PARK7 which close related with the Pathogenesis of pituitary adenoma,CA1 is possible suppressor factor,and ESD maybe have the potential of clinical application. Onset of pituitary adenoma is complex process, which a multiple factor and multi-step participate in. Partâ…¡Create the total proteins database of human normal pituitary tissue by 2D-HPLC combined with LTQ Orbitrap ESI MSObjective Create the total proteins database of human normal pituitary tissue.Methods Analyze nomal pituitary tissue by 2D-HPLC combined with LTQ Orbitrap ESIMS,use shotgun to quantitate the ptotein,then create the total proteins database.Result Succesfully create and classify total proteins database of human normal pituitarytissue within 406 protein dots. The highest proportion of proteins database is proteinswhich have the function of binding and cohere(41 %),proteins which have catalytic activitytake the second place(28 %).Among them, important proteins take large content asfollow.xollagen protein IV, prolactin, somatotropin and microtubule.Conclusion The latest Proteomics technique applying can be efficient, accurate andcomprehensive establish the total proteins database of human normal pituitary. That createthe total proteins database of human normal pituitary tissue is significant. It fill the blank ofpituitary study by proteomics methods, and it aslo lay the foundation for the furtherinvestigation about the pathogenesis of pituitary adenoma.