Selection Of HFRSV-specific Epitopes By Screening Random Peptide Libraries With HFRSV-positive Sera

Objective:To obtain the 12-mer phage clones displaying the Hantaan virus mimic epitopes.Methods:HFRSV-positive sera were used as selective molecules for biopanning.A 12-mer phage peptide library was bio-panned for 5 rounds,and the result was confirmed by sandwich ELISA,competition ELISA and DNA sequencing.Results:After 5 rounds of effective screening,the results detected by sandwich ELISA and competition ELISA revealed that majority of the selected clones could react to positive sera in a dose-dependent manner,whereas they could not bind to BSA and the control sera.Sequencing and alignment analyses indicated that amino acid sequences of 45 positive clones could divide into eight groups,and seven of them revealed putative motifs:LVXKR,LTXR,IXKP,LXPA,VGA,KXIR and EKXP.Four of the putative motifs had a homologous region within structural proteins of HFRSV.Conclusion:The peptides displayed by phage can mimic the epitopes of HFRSV antigen,which provides the potential for preparing more effective epitope-based vaccine and specific diagnosis reagent.