Expression And Clinical Significance Of CDC25B And Aurora-A In Human Non Small Cell Lung Cancer

Background: Recently CDC25 family, which is the upstream phosphatase of CDK1 is significantly noticed by many scientists. The CDC25B phosphatases are involved in the dephosphorylation and activation of CDK-1 at the key cell cycle transitions. CDC25B acts as an initiator of the early mitotic events. Aurora-A, as a member found in recent years belonging to serine/threonine kinase family, proves relating to centresomes'maturation and chromosomes division. It has been confirmed as a new oncogene due to its high expression in multiple cancers cells, playing a key role in the normal process of mitosis as proved in previous researches. Aurora-A activity is required for the recruitment of CDK1-cyclin B1 to the centrosome prior to its activation and thecommitment of the cell to mitosis. CDC25B phosphorylation is dependent on Aurora-A. Recent researches found that Aurora-A and CDC25B over expresses in various malignancies.Objective: To explore the relationship between the expression of CDC25B or Aurora-A and the clinic pathological features of no small cell lung cancer. Provide a theoretical basis for screening tumor marker and molecular therapeutic target of lung cancer.Methods: Using SP immunohistochemistry methods, the expression of CDC25B and Aurora-A in 76 cases of non small cell lung cancer and 11 cases of benign lung disease was detected. The relationship between the expression of CDC25B or Aurora-A and the clinic pathological features of non small cell lung cancer was analyzed by the statistical method. Aurora A and CDC25B mRNA and protein expression were examined by reverse transcription–polymerase chain reaction (RT-PCR) and Western blot respectively.Results: In this research, over expression of CDC25B was found in 43/76 patients (56.9%) compared with their corresponding neighboring tissue (2/11, 18.2 %) through immunohistochemistry. Over expression of Aurora-A is 64.6% and 22.4% .with significant difference shown byχ2 test under SPSS 11.5(p﹤0.01). Implying the accordance in Aurora-A or CDC25B protein expression level between the result a from Western bolt and that from immunohistochemistry. In this research, the expression of Aurora-A and CDC25B in tumor in vivo from 30 cases of lung cancer patients was detected through RT-PCR. Over expression was observed in 19 cases (63.3%) /in 17 cases (56.7%) compared with corresponding neighboring tissues. as proven by Bandleader Analysis and Paired t-test under SPSS 11.5(p﹤0.05).Conclusions: Over expression of Aurora-A and CDC25B mRNA/protein is presents in vitro of non small cell lung cancer. Furthermore the over expression of Aurora-A and CDC25B is compact correlation. And both Aurora-A and CDC25B are all useful factors in predicting prognosis of patients with non small cell lung cancer.