Gene Single Nucleotide Polymorphisms Of VEGF And The Risk For Polycystic Ovary Syndrome

Objective:Polycystic ovary syndrome(PCOS)is a common and frequently encountered dysfunctional disease in endocrine of women.Its development is primarily controlled by inheritance.Most cases of this disease are proved to be autosomal dominant inheritance.Vascular endothelial cell growth factor(VEGF) is an angiogenic factor and it exists in five isoforms,VEGF-A,VEGF-B,VEGF-C,VEGF-D and placental growth factor(PLGF).Through three tyrosine kinase receptors VEGFR-1/ Fit-1,VEGFR-2/KDR and VEGFR-3/Flt-4 VEGF acts as a stimulator for vascular endothelial cell proliferation and as a regulator for vascular permeability.It has been suggested that VEGF may play a role in ovarian angiogenesis and in the regulation of vascular permeability.To investigate single nucleotide polymorphisms(SNPs) in VEGF gene that have significant associations with the PCOS.The aim of our study was to investigete the frequency of SNPs in VEGF gene at site +9812 and +13553 in the han nationality its role in the pathogenesis of the PCOSMETHODS:80 cases of patients with PCOS and 84 cases of health control women random selected were studied.Body mass index(BMI) and waist- to- hip ratio(WHR) were determined.Blood samples were obtained for DNA analysis and hormone testimetes.Sequecing analysis of SNP at +1982 and +13553 and follicle counting of diovary were performed.RESULT:We found that SNP at site +9812 CG genotype and G allele have significant difference(P＜0.05).there was no significant difference in the frequency of VEGF+9812 CC/CT genotype and at site+13553 CC/CT genotype between PCOS and healthy controls(P＞0.05).Comparision of two sites among Afirican Europen korean and healthy controls was significant difference(P＜0.05).The group of PCOS had significan -tly difference T,LH,LH/FSH,E2,P,Fc levels than those of the controls(P＜0.05),but FSH and PRL no significant different(P＞0.05).Conclusion:We concluded that SNP at+9812 site have a high possibility of significant associations with the pathogenesis of PCOS.CG may have protective on the pathology of PCOS SNP at +13553 site did not contribute to PCOS Two sites is different among populations.