The Effect Of HSV-1 Infection On The Expression Of HnRNP H2 In Human Liver Cell

Herpes virus, as a kind of important pathogen for human, can induce various diseases. Herpes simplex virus (HSV) that is one group of this family induces popular infection in human. In its primary infection, HSV, which is able to induce the specific immune response of individual, can keep its long time existence in neurons of host through latent state and will be reactivated under some certain conditions to induce pathological damages in epidemic tissues.HSV-1 infected cells present a serial changes in micromolecular metabolism, especially in protein synthesis pathway, including degradation of cellular mRNA, shutoff host transcription, selective stabilization and activation of cellular protein, and so on. By used the proteomics platform, normal human liver cell line L-02 and HSV-1 infected L-02 cellular protein mixture were separated individually by 2-DE. A set of protein spots in 2-DE pattern were matched. In this research, it found cellular protein heterogeneous nuclear ribonucleoprotein H2 (hnRNP H2) decreased in HSV-1 infected L-02. hnRNP H2 is a positive regulator in pre-mRNA processing. The changed expression level of this proteins in HSV-1 infected cell may imply that all of them play important roles involving in virus-host interactions to affect the protein expression pattern. in order to verification The effect of expression in different stage of virus infection, we use the Northern blot and Western blot technologies. To further explore the function of the significant splicing protein-hnRNP H2 in the virus infection and interaction with viral protein.By analysis the result, we can draw a conclusion that maybe the participate of the IE expression product, which dramatically effect the expression of hnRNP H2.The changed expression level of this protein in HSV-1 infected cell may imply that all of them play important roles involving in virus-host interactions to affect the protein expression pattern and at the same time will offer us a new clue to analysis the changes of host cell after the infection of virus.