Preparation And Characterization Of Gelatin-Siloxane Nanoparticles As Gene Delivery System

The main objective in gene therapy is the development of efficient, non-toxic gene carriers. Non-viral vectors provide opportunities for improved lower toxicity, non-pathogenic and more facile manufacturing. In this work, the new hybrid nanoparticles with organic-inorganic contents of gelatin and siloxane were successfully synthesized. We described the synthesis of gelatin-siloxane nanoparticles and tested these vectors based on those NPs as vectors for gene therapy. The main results are as follows:1. Preparing gelatin-siloxane nanoparticles by a sol-gel process in gelatin, GPSM, ammonia system or gelatin, GPSM, APTMS system. The synthetic scheme was also studied.2. A systemic study of nanoparticles/DNA complexes in gene therapy was conducted. The complexes with nanosized diameters could protect DNA from digestion in phosphate buffer solution (pH 7.0 and pH 6.0), serum and DNase I solution.3. The strategic to improve transfection ability of gelatin-siloxane nanoparticles comprise functionalizing the surface of the NPs with biofunctional peptide. A systemic study of peptide-NPs/DNA complexes in gene therapy was conducted. The peptide-NPs with higher surface charges and nanosized diameters could protect DNA from digestion in phosphate buffer solution (pH 7.0 and pH 6.0), serum and DNase I solution.