| With the development of modern medicine, cancer has gradually become the most important fatal human disease, with the continuous development of science and technology, the detection of cancerous tissue and other related anti-cancer drug being attached great importance to science.The traditional treatment of cancer patients give a great deal of physical and mental trauma, with the result that people are constantly looking for a safer, convenient and effective treatments to alleviate the suffering of the sick and the continuation of a better life. Small molecule anti-cancer drug′s development will not only allow people to see hope, but also deep into the traditional methods in the past it will not be effective treatment of leukemia, lymphoma and skin cancer and other fields.Based on small molecule anti-cancer drugs in a variety of pharmacological research, but also in the DNA, protein levels have focused on normal cells and cancer cells to identify and deepen people's understanding of cancer and enhance the clinical diagnosis of early cancer and to guide the development of anti-cancer drugs. Raman spectroscopy detection techniques because of non-destructive, non-invasive, high resolution, reagents and do not have the advantages of high automation importance by the people.If cancer can be early detected, cure rates will up to 80 percent.This is the latest authoritative conclusions of the World Health Organization. In the early diagnosis of cancer, because of the lack of specificity of the individual patient data itself and the noise and other factors, to be very accurate diagnosis is difficult. There are three main types of modern medicine methods to detect cancerous tissue, blood-chip method, polymerase chain reaction method and nano-detection technology.The three common form of cancer detection technology are varying degrees of defects, Cancer Detect chip drop of blood can only be targeted to a particular system and should not determine in a specific organ, the final diagnosis needs other Detect Cancer method. Means of PCR have very high technological content, the same skill level required to operate the professionals, nano-technology is only used for laboratory testing, not for clinical, the world's scientists are working to this technology in clinical medicine.Research on biological macromolecules, especially nucleic acid and protein molecules and the interactions between small molecules, in the medical, pharmaceutical and biological fields is of great significance. Nucleic acid organisms are an important component of the material, the carriers of genetic information and gene expression of the basic material, it plays an important role at the growth, development and reproduction of such activity. For example, the majority of anti-cancer drugs into the body are based on DNA as a target, and therefore the study of small molecules and the role of DNA in the anti-cancer drugs, in particular, anti-AIDS drug design and development work with a pivotal position can be targeted for drug synthesis and provide a theoretical basis, so that the role of production by way of reservation in a specific location in the DNA of the anti-cancer drugs has become possible to reduce the toxicity of anticancer drugs . Similarly, the protein is also important in vivo physiological function of macromolecules, serum albumin is the most abundant plasma proteins, with many endogenous and exogenous compounds combine to play the role of storage and transport, through research albumin and drug molecules can know drug in the body, as well as the transport mechanism. DNA are essential to life's genetic material, DNA analysis and quantitative molecular identification of specific genomics, virology, molecular biology and other related subjects of great significance to the development. Molecular biology because their fluorescence is weak, a few days ago the use of fluorescent probe assay. Fluorescent probe detection of more traditional isotope speed, good reproducibility, with less than kind, no radiation, in the automatic DNA sequencing, antibody analysis, disease diagnosis, analysis of anti-cancer drugs has been widely used, DNA fluorescence probe detection sensitivity is the result of the impact of the important factors, thus the development of more sensitive fluorescent probe, while avoiding interference from background fluorescence in biology has become a hot research.Fluorescent probe technology, with high-sensitivity, high specificity and high accuracy characteristics. Are widely used in flow cytometry, immunoassay, molecular biology, cell analysis and apoptosis of tumor cells to identify areas of research, etc.Modern scholars in the observation of tumor growth and proliferation of non-conforming, it was found that with the cell cycle regulation-related protein, and that the tumor cell cycle regulation and is closely related disorders. Cyclin cell cycle regulators as one of a variety of over-expression are characteristic of human primary tumor, the tumor diagnosis and prognosis of great significance. Scientists are finding in the study, almost all cancer genes, the biological effects of tumor suppressor gene, are finally brought together up to the cell cycle machinery, many oncogenes, tumor suppressor genes directly involved in cell cycle regulation or cell cycle regulation itself is complex the main components. The results of these gene mutations, leading to uncontrolled cell cycle. Out of control unrestricted access to the cell proliferation is the formation of tumor cloning groups. Therefore, some scholars think is a kind of tumor cell cycle disease. Three Nobel Prize-winning scientists at tumor diagnosis of the basic work to make possible for the treatment of cancer has opened up new avenues.The first part of this thesis is the use of UV absorption spectrum of such a common means of spectral analysis of chemical drug ethidium bromide (EB) and the interaction between DNA analysis of EB is a planar molecular structure of the Philippine PI fluorescent dyes, can be inserted into the base pairs of double-stranded DNA occurred in the role. Its own fluorescence weak, but inserted into the DNA base pairs can cause a significant enhancement of fluorescence intensity, about 100 times enhanced. Usually use it to study the interaction between drugs and DNA EB inserted between base pairs of DNA, so that the original DNA helix closer to relax, if drugs similar happened with the DNA of the role of drug molecules bound with bromine bromide of competition and the DNA binding site, so that EB from the DNA, so that the system reduced the fluorescence. Study on EB so with the interaction between the DNA is very important.EB successive solution to add a certain amount of DNA so that DNA content of one of10μmol /L,20μmol /L,40μmol /L。 Interface to four curves can be seen to enlarge EB solution with single-EB +20μmol /L DNA, EB +40μmol /L DNA delivery in a very good point, and EB +10μmol /L DNA and single-EB solution appears at the bottom point of intersectionWhen the EB +10μmol /LDNA (red) line on the overall shift in the single-EB solution and EB +20μmol /LDNA, EB +40μmol /L DNA and found the whole intersection curve has a large red shift, beyond the experimental error scope.Vertex Department for the entire curve shifted up (red) a high level of 2.7% in the experiment because of the increase in DNA are the successive curves can be and after the two curves (EB +20μmol /L DNA, EB +40μmol /L DNA) make gradual analysis.EB, as well as through the analysis of DNA structure we have come to insert mode EB molecules attached to the junction of purine and pyrimidine DNA damage in hydrogen bonding, but no serious impact on DNA skeleton and an EB molecule with two different DNA bonding between the chains this conclusion.The second part of this thesis is to seek FITC fluorescence probe such as Raman probe the feasibility of, and the use of small peptides with CyclinA to the characteristics of specific binding using FITC labeled Cyclin A small peptide of conduct Quantitative analysis of mathematical models.Through constantly adjust and change as well as the laser power increases the concentration of silver sol by the FITC-peptide can be used as the conclusions of the Raman probe, and FITC-peptide obtained in silver sol and the ratio of about 1:20 the best this conclusion. On this basis, we have studied the Cyclin A and how to proceed with FITC-peptide bindingCyclin A analysis of FITC-peptide with the C-C bond, C-N covalent bond to the combination, with constant CyclinA add FITC-peptide characteristic of the Raman peak intensity also decreased. Obtained by calculating the quantitative detection of Cyclin A mathematical model of surface-enhanced Raman spectroscopy with the FITC-peptide in the cyclin Cyclin A study shows that the interaction between the Raman spectra of cancerous tissue in the diagnosis of a bright future, Raman spectroscopy study of their interaction can not only provide a basis for diagnosis of cancerous tissue, and for cyclin target Cyclin A for anti-cancer drug research possible, if the anti-cancer drugs with DNA, the role of protein combined with research, there is no doubt this will be the mechanism of anti-cancer drugs know the better method of screening for anti-cancer drugs to provide a new idea.
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