The Extraction Of Effective Constituents From Viola Philippica Car. And Quality Standard Study

Viola philippica Car. is a perennial herb of dicotyledon class. It also has some synonyms. Viola philippica Car. originates from China. It is widely distributed in Liaoning, Hebei, Henan, Shandong, Shanxi, and also in North Korea, Russia and Japan.Viola philippica Car. is a traditional Chinese medicine, which is described in Chinese medicine with bitter, spicy, cold characters. It has many functions, like heat-clearing, detoxifying, blood-cooling and detumescence. For internal use, it is usually compatible with lonicera japonica, forsythia and chrysanthemum indicumin, in order to cure urinary tract infections, dysentery, mastitis, conjunctivitis, pharyngitis etc; for external use, mashed fresh Viola philippica Car. can treat sore, carbuncle, traumatic injury, erysipelas, cellulites and snakebite. This plant has a variety of chemical compositions, such as flavonoids and their glycosides, coumarins, organic acid, microelement, etc. Studies show that Viola philippica Car. has functions of anti-flammatory, immunoregulation, anti-oxidation and anti-HIV. Clinically, it has been also used for tonsillitis, acute suppurative inflammation in skin, respiratory system disease, thrombus and AIDS. Now, ointment, suppository and tablets for Viola philippica Car. are commercially available.Nowadays, with dramatic development of separation and spectroscopy technology, studies on constituents and bioactivity of traditional medicine is advanced. The chemical study of Viola philippica Car. is not systematic for now, and most researches in efficient constituents extraction and production processing are still in experimental phase. Viola philippica Car. has a wide clinical application, but the mechanisms of the process are not manifested. The relationship between curative effect and type, quantity, pharmacological action, targets of constituents is not clear. All above are restrictions of the further developments of Viola philippica Car. Exploration of Viola philippica Car. has been largely limited, since no unified quality standard has been established. Therefore, it is vital to combine the identification of traditional medicine with chemical and pharmacological methods to find out representative active constituents of Viola philippica Car.. Establish the quality standard of herb and praeparatum as the fundament for development of Viola philippica Car..The content of this reseach is as follows:1. Summarize the chemical constituents, pharmacological action and clinical application of Viola philippica Car..2. Isolate three compounds from Viola philippica Car., which are identified as 6,7-Dihydroxycoumarin(esculetin), 7-hydroxy-6-methoxycoumarin(scopoletin) andβ-dauco-sterol (daucosterol) by physical, chemical methods, spectroscopy constants and documents comparison. Daucosterol is first extracted from this herb.3. We established a technique to extract esculetin, by which, we can get compound form of esculetin in a rapid and convenient way. It provides a fundament for industrialization of standard substance.4. Quantitate of esculetin by HPLC also provides experimental support for establishment of unified quality standard.