Enhanced Cellular Immunity Induced By CD40L Activated Dendritic Cells In CEA Transgenic Mice

Objective:Gastrointestinal caners are the most common types of malignant tumors in Human Beings.The main treatment remains surgery resection,in addition to which,adjunctive therapies are far not satisfactory for the target of which are not specific.Dendritic cells cancer vaccine is one of the most promising immunotherapy ways developed in the decades. CD40L is a key molecule in the maturation of dendritic cells.Our research is designed to enhance the cellular immunity which is induced by CD40L activated dendritic cells in CEA trasnsgenic mice.And find out the efficacy of using CD40L to activate dendritic cell and inducing specific cellular immunity.Method:We use the bone marrow cells of the CEA transgenic mice to generate immature dendritic cells under the condition of GM-CSF and IL-4.CD40L is added to activated dendritic cells into mature phenotype.Dendritic cells cancer vaccine is puled with CEA_526-533 peptide which makes the vaccine specific for cancer immunity.The immunophenotype molecules are identified by flow cytometry.The cytokines produced by cells are determined by ELISA.T cells proliferation is measured by ³H-thymidine essays.Result:1.Immunophenotype molecules expression by CD40L activated dendritic cells are higher than un-stimulated DC.2.IL-12 secretion by CD40L activated dendritic cells is higher than un-stimulated DC (P＜0.01).3.CD8⁺ T cells proliferation induced by CD40L activated dendritic cells is stronger than un-stimulated DC(P＜0.05),while the secretion of INF-γis higher as well(P＜0.01).4.Splenocytes proliferation induced by CD40L activated dendritic cells is stronger than un-stimulated DC(P＜0.01),while the secretion of INF-γis higher as well(P＜0.01).Conclusion:The method of using CD40L to stimulate bone marrow delivered dendritic cells promotes the maturation and activation of dendritic cells,which enhance the cellular immunity in CEA transgenic mice.