Ovarian Recovery After Radiotherapy By Bone Marrow Transplantation

With rapid development in the field of tumor radiotherapy,the fidelity of prognosis and surviving rate of patients with cancer improved obviously.According to the statistical results from 1995～2005 in U.S.A,the survive rate of the infiltrating carcinoma patients reached as high as 60%.With the improvement of long term survive rate of young female patients,more and more attention was paid to iatrogenic harm.For example,long term physiological and psychological hurt.One of long term complication in radiotherapy is ovary toxicity.This is due to the finity of ovary germ cells,which can't be regenerated.Therefore,to many young female patients who expect normal pregnant,the sterility and early menopause caused by radiotherapy affect their life quality and self-confidence seriously.Recently,the study on the mechanisms and prevention of ovary toxicity caused by radiotherapy aroused the interest of researchers in the field of phymatology,reproductive medicine,radiative prevention.This paper initially studied the feasibility and mechanisms of the recovery of radiotherapic ovary damage by marrow transplantation.The aim of this paper is to determine the recovery level of radiotherapic ovaries after marrow transplantation by observing pathological morphous,testing FSH,E₂ level in blood serum of mouse and checking whether estrous cycle was reoccurred. Then,after observing the differences between homogeneity marrow transplantation group(HOMT) and heterosexual(HEMT) marrow transplantation groupto,a new mechanism for ovary recovery can be provided. The study was divided into three parts.The first part mainly studied the fuction of ovary recovery by transplanting marrow into radiotherapic mouse;the second part discussed different ovary recovery levels between HOMT and HEMT;the third part initially explained the possible mechanism of recovery in this way.1.In the first experiment,we used 140 8～10 weeks(w) Kunming female mouse(SPF,same in the following experiments),20 as transplant donor,40 as control group which didn't receive any treatment;40 as radiotherapy group which were radiated once;40 as transplant group which were performed marrow transplant within 24h after radiation.On the day 1^st,5^th,15^th,30^th we selected 10 mouse from each groups respectively to detected the E₂,FSH in blood serum,weighted their ovary index and made pathological section of ovary.2.In the second experiment,we used 175 SPF 8～10w Kunming female mouse and 15 SPF 8～10w male Kunming mouse.15 male mouse as transplant donor and 15 female mouse as transplant donor,40 as control group which didn't adopt any treatment;40 as radiotherapy group which radiated once only;40 as transplant group which performed female marrow transplant within 24h after radiation,40 as transplant group which performed male marrow transplant within 24h after radiation. On the day 1^st,5^th,15^th,30^th we selected 10 mouse from each groups respectively to detected the E₂,FSH in blood serum and weighted ovary index and made pathological section of ovary.3.In the third experiment,we used 22 female ICR mouse,5 female ICR fluorescent mouse.6 ICR mouse as radiotherapy group which radiated only once,6 ICR mouse as control group which didn't have any treatment,5 fluoresent mouse as marrow transplant donor,10 ICR as marrow transplant group which transplant fluorescent marrow within 24h after radiation.On day 15^th,30^th,we select half of the group perpform ovary frozen section respectively. In the experiment on whether marrow transplant is good for ovary recovery harmed by radiotherapy,on day 15^th and 30^th,we observed marrow transplant group reappeared more primordial follicle,primary follicle,secondary follicle and mature follicle compared with radiotherapy groupand,and the vessels in ovary was comparatively abundant.The number of ovum in radio therapy group was dropped dramatically.The E₂ and FSH level in blood serum and ovary index between radiotherapy group and marrow transplant group has significant differences on day 15^th and 30^th.(The F value of E₂,FSH,ovary index on 15^th day was 15.676,2.854, 27.215 respectively,the P value of E₂,FSH,ovary index was 0.000,0.000,0.045 respectively;The F value of E₂,FSH,ovary index on 30^th day was 12.610,15.657, 19.709 respectively,the P value of E₂,FSH,ovary index was 0.000,0.000,0.000).In the experiment on whether marrow from different sex is different for ovary recovery harmed by radiotherapy,on day 15^th and 30^th,compared with radiotherapy group,we observed female marrow transplant group reappeared primordial follicle, primary follicle,secondary follicle and mature follicle,the vessels in ovary was comparatively was abundant.The number of ovum in radiotherapy group and male marrow transplant group was dropped dramatically.The E₂ and FSH level in blood serum and OI between radiotherapy group,HEMT and HOMT had significant differences on day 15^th and 30^th.(The F value of E₂,FSH,OI on 15^th day was 124.038,95.431,15.346 respectively,the P value of E₂,FSH,ovary index was0.000, 0.000,0.000 respectively;The F value of E₂,FSH,OI on 30^th day was 180967,190.495,10.968 respectively,the P value of E₂,FSH,ovary index was0.000, 0.000,0.000)In the third part,we found GFP cells in the GFP marrow transplantation group on d15 and d30,while in other groups we didn't found any GFP cells.According to the results above,a conclustion can be drawed that marrow transplantion of same sex contributes to the recovery of ovary harmed by radiation. The marrow cells from extrinsic sourc have effect on both ovary itself and can be further developed into ovums.