The Study Of Urokinase+ Low Molecular Weight Heparin(LMWH) On Vascular Morphological Changes Of Acute DVT In Rats

Objective: To observe the high-dose anticoagulant therapy plus thrombolysis of acute DVT rats at different times after treatment of vascular morphological changes.Methods: 48 SD model of rats, according to Random thrombosis days after 1, 3, 7, 14 phases, each lasting 12, is divided into stageâ… ,â…¡period,â…¢period,â…£period. Urokinase (80, 000 U / kg ? d, n = 6) + low-molecular-weight heparin (700 U / kg, a / 12 h, n = 6), the model group (0 U / kg, n = 6). UK daily rat tail intravenous injection of urokinase in a row on the 7th. Low-molecular-weight heparin by injection in the abdominal wall, a row on the 7th. After the acquisition of vascular lesions observed changes in the number of endothelial cells, collagen positive staining of percentage change, and with the same period in the model group for comparison. Results: The model group in the stageâ…¡,â…¢period were a death, urokinase + low-molecular-weight heparin in the group stageâ…¢thrombolytic therapy, a death.â… in the period, low-molecular-weight heparin urokinase + endothelial cells, collagen positive staining area percentage compared with the model are significant differences (P < 0.05).â…¡in the period, urokinase + low-molecular-weight heparin Group endothelial cells compared with the model there was a difference (P<0.05), urokinase + low-molecular-weight heparin group collagen positive staining area percentage compared with the model are significant differences (P<0.05) .â…¢in the period,â…£period, urokinase + low-molecular-weight heparin group endothelial cells compared with the model group had no statistical difference (P>0.05); urokinase + low-molecular-weight heparin group collagen positive staining area Percentage compared with the model had no statistical difference (P > 0.05). Conclusion: 1. Endothelial cells as to evaluate the effect of DVT treatment: high-dose anticoagulant thrombolysis increase in thrombosis after three days of treatment have a certain effect in thrombosis within seven days of high-dose thrombolysis plus there are still some anticoagulant Thrombolytic effect more than seven days, plus high-dose anticoagulant thrombolysis alone or thrombolytic, anti-coagulation is void. 2. PTS to the development of collagen as an evaluation of the results: high-dose anticoagulant thrombolysis increase in thrombosis after three days of treatment have a certain effect in thrombosis after seven days, plus high-dose anticoagulant thrombolysis is still a certain thrombolytic Effect over seven days, plus high-dose anticoagulant thrombolysis alone or thrombolytic, anti-coagulation is void. 3. If the endothelial cells and collagen as a whole, high-dose anticoagulant in DVT thrombolysis increase the incidence of early treatment is best. 4. Thrombolytic joint clinical anticoagulant treatment pending further study.