The Expression And Relativity Between Co-stimulative Molecule B7-H3 And Human UDP-Gal: BetaGlcNAc Beta 1, 3-galactosyltransferase Polypeptide 7 In Malignant Tumor

Object: To study the expression and clinical significance between co-stimulative molecule B7-H3 and human UDP-Gal: betaGlcNAc beta 1, 3-galactosyltransferase polypeptide 7 in malignant tumor and explore the relativity between Co-stimulative Molecule B7-H3 and Human UDP-Gal: betaGlcNAc beta 1, 3-galactosyltransferase Polypeptide 7.Methods: The 96 cases with radical surgery were the paitions of the fourth People's Hospital in Wuxi from 2005 to 2007.The expression of B7-H3 and GalT7 were detected with Immunohistochemistry.Several online softs were used to predicted glycosylation site in B7-H3 sequence. Co-immunoprecipitation was used to study the combination of B7-H3 protein andβ3GalT7 (GnT8) protein.Immunofluorescence was used to locat the two proteins.Results: The expression of B7-H3 and GalT7 is intimately related to lymph node metastasis in the malignant tumor, and independent of age and sex. There are 3 O- glycosylation sites, 8 N- glycosylation sites and 18 O-(beta)-GlcNAc sites in 4IgB7-H3 sequence. B7-H3 protein andβ3GalT7 (GnT8) can combine together and mutual locate in cell.Conclusion: The expression of 4IgB7-H3 andβ3GalT7 (GnT8) was positive correlation with lymph node metastasis in the malignant tumor. B7-H3 protein andβ3GalT7 (GnT8) may interact by analyzing sequence and location.β3GalT7 (GnT8) may modify 4IgB7-H3 protein.