The Effect Of IL-15 On Pathogenesis Of Rheumatoid Arthritis

IL-15(Interleukin-15) was found by Grabstein in 1994. It stimulates the proliferation of T lymphocytes and shares many biological properties with IL-12, and is produced by a wide variety of tissue. Several studies show that IL-15 played an important role in pathogenesy of rheumatoid arthritis. we studied the pathological change of synovial membrance in the collegen induced arthritis rats, and the relationship between IL-15 and the other cytokines(including IL-15 and TNF-α),so that we could infer the effect of IL-15 on the pathogenesy of RA.PartⅠ. The effect of IL-15 on the pathological change of synovial membrane in the Collagen induced arthritis (CIA) ratsPurpose The study was performed to explore the effect of IL-15 on the pathological change of synovial membrane in the Collagen induced arthritis (CIA) rats. Methods 60 wistar rats were randomly divided into four groups (including control group A, control group B, model group A and model group B). The Collagen induced arthritis (CIA) rats model were prepared in the model groups by CollagenⅡemulsion. IL-15 was injected into the ankle of rats in the control group A and the model group A in the fourth week, and liquor natrii chloridi isotonicus was injected into ankle of rats in the control group A and the model group A. The pathological change of the joint were observed in two weeks after injecting. Results the degree of arthrocele was more serious in control group B, model group A and model group B than those in control group A(p<0.05). Furthermore, it was more serious in model group A than those in control group B(p<0.05). There was significant difference in the degree of arthrocele between the model group B and model group A. Pathologic accumulated points of the pathological change of the joint was higher in control group B, in model group A and model group B than those in control group A(p<0.05). Meanwhile, the difference between model group A and control group B was to be significant. There was significant difference in the accumulated points between model group B and model group A. Conclusions It was concluded that IL-15 showed the effects on mediators of joint destruction, synovial membrane hyperplasia in RA, which was an important factor in the cause of RA.PartⅡ.The relationship between IL-15 and the other cytokines1. The effect of IL-15 on the change of TNF-αexpressing in peripheral blood and synovial membrane in the Collagen induced arthritis (CIA) ratsPurpose To study the effect of IL-15 on the change of TNF-αexpressing in peripheral blood and synovial membrane of the Collagen induced arthritis (CIA) rats. Methods 120 wistar rats were randomly divided into two groups(including groupⅠa nd groupⅡ). The Collagen induced arthritis (CIA) rats model were prepared by CollagenⅡemulsion in two groups. IL-15(content recombinated rat IL-15 1600μg) was injected into the ankle of rats in the the model groupⅡin the secornd week. Likewise, the rats in the model groupⅠwere treated with liquor natrii chloridi isotonicus. Detecting TNF-αin peripheral blood and synovial membrane of the Collagen induced arthritis (CIA) rats were in eight weeks after injecting. Results The level of TNF-αin peripheral blood and synovial membrane was higher in the groupⅠt han those in groupⅡin different periods(p<0.05). The change of TNF-αin every periods is more obvious in groupⅠt han in groupⅡ.Conclusions It was concluded that IL-15 was able to promote the peripheral blood and synovial membrane to express TNF-α, especially in the initial stage of RA. In the advanced stage, IL-15 can sustain the expressing of TNF-αin the synovial membrane of CIA rats.2. The effect of IL-15 on the change of IL-17 expressing in synovial membrane in the Collagen induced arthritis (CIA) rats and the effect of MTX on the two cytokines.Purpose To study the effect of IL-15 on the change of IL-17 expressing in synovial membrane in the Collagen induced arthritis (CIA) rats and the effect of MTX on the two cytokines. Methods 58 wistar rats were randomly divided into five grooups (including control group, model groupⅠ, model groupⅡ, model groupⅢa nd model groupⅣ). The Collagen induced arthritis (CIA) rats model were prepared by CollagenⅡemulsion in the model groups. IL-15 was injected into the ankle of rats in the model groupⅡ, the model groupⅢand the model groupⅣin the secornd week. Meanwhile, the rats in the control group and the model groupⅠwere treated with liquor natrii chloridi isotonicus. The model groupⅢwas intraperitoneal injected MTX 0.25mg·kg-1 and the model groupⅣwas intraperitoneal injected MTX 0.5mg·kg-1every three days. IL-17 expressing in synovial membrane in the Collagen induced arthritis (CIA) rats were observed in two weeks after injecting. Results The level of IL-17 in synovial membrane was higher in model groups than those in control groups(p<0.05). Furthermore, the difference between model groupⅡand model groupⅠwas to be significant. There was significant difference in the level of IL-17 between model groupⅡand model groupⅢ. It was higher in model groupⅡthan in model groupⅣ(p<0.01).Conclusions It was the conclusion that the synovial membrane of CIA rats was able to express IL-17 and IL-15 can promote the synovial membrane to express Il-17. But the MTX can inhibit this effect of IL-15.